TAILIEUCHUNG - báo cáo khoa học: " Overexpression of Pim-1 in bladder cancer"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Overexpression of Pim-1 in bladder cancer | Guo et al. Journal of Experimental Clinical Cancer Research 2010 29 161 http content 29 1 161 RESEARCH O1 It I Journal of Experimental Clinical Cancer Research Open Access Overexpression of Pim-1 in bladder cancer 1t 1t 1 1 23 24 1 Shengjie Guo Xiaopeng Mao Junxing Chen Bin Huang Chu Jin Zhenbo Xu Shaopeng Qiu Abstract Background Pim-1 is a serine-threonine kinase which promotes early transformation cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types however the function of Pim-1 in bladder cancer is poorly understood. Methods Expression and localization of Pim-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and Pim-1 staining score was compared with several clinicopathologic parameters. To further demonstrate the biological function of Pim-1 in bladder cancer its expression was validated in five bladder cancer cell lines by western blot and immunohistochemistry analyses. Subsequent knockdown of Pim-1 was achieved by lentivirus encoding small interfering RNA and the effect of Pim-1 on bladder cell survival and drug sensitivity were further assessed by colony formation and cell proliferation assays. Results When compared with normal epithelium Pim-1 was overexpressed in bladder cancer epithelium and the expression level was higher in invasive bladder cancer than Non-invasive bladder cancer specimens. Pim-1 was also detected in all the bladder cancer cell lines examined in our study. Moreover the knockdown of Pim-1 significantly inhibited bladder cancer cell growth and also sensitized cells to chemotherapeutic drugs in vitro. Conclusions Our results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance Pim-1 is a potential candidate for targeted therapy in bladder cancer. Background Bladder cancer .

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