TAILIEUCHUNG - Báo cáo y học: " Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:" Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD. | Lofdahl et al. Respiratory Research 2011 12 48 http content 12 1 48 RESPIRATORY RESEARCH RESEARCH Open Access Tenascin-C and alpha-smooth muscle actin positive cells are increased in the large airways in patients with COPD Magnus Lofdahl 1 Riitta Kaarteenaho2 Elisa Lappi-Blanco3 Goran Tornling1 and Magnus C Skold1 Abstract Background Chronic obstructive pulmonary disease COPD is characterized by inflammation and remodeling of the lungs. This results in alterations in extracellular matrix ECM and structural changes leading to airflow obstruction. We studied the expression of tenascin-C Tn-C and alpha smooth muscle actin a-SMA which act as a marker of myofibroblasts in large airways from COPD patients. Our aim was to elucidate whether this expression correlated with smoking or with disease development. Methods Bronchoscopy was performed on 20 COPD patients mean age 56 years range 39-61 FEV1 FVC 70 and FEV1 median 53 range 33-69 of predicted . Age and smoking matched smokers S without COPD n 13 and age matched non-smokers NS n 14 served as controls. Bronchial mucosal biopsies were analyzed by immunohistochemistry. The distribution of Tn-C expression was assessed and graded in three levels and the number of spindle shaped cells staining positive for a-SMA were counted. Results Biopsies from COPD patients had more P Tn-C expression than the two control groups. A significantly P increased number of spindle shaped cells expressing a-SMA was observed in COPD patients compared with the controls. Smokers and nonsmokers did not differ in this respect. The expression of Tn-C correlated positively P to the number of a-SMA positive cells. Conclusions We demonstrate increased expression of Tn-C and a-SMA positive cells in the large airways in COPD. This was not associated to smoking per se but to the presence of airway obstruction. Our findings add new information regarding remodeling characteristics and highlight the large airways as a .

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