TAILIEUCHUNG - Báo cáo y học: "Arthritis is associated with T-cell-induced upregulation of Toll-like receptor 3 on synovial fibroblasts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Arthritis is associated with T-cell-induced upregulation of Toll-like receptor 3 on synovial fibroblasts. | Zhu et al. Arthritis Research Therapy 2011 13 R103 http content 13 3 R103 RESEARCH ARTICLE Open Access Arthritis is associated with T-cell-induced upregulation of Toll-like receptor 3 on synovial fibroblasts f f 3 4 Wenhua Zhu Liesu Meng Congshan Jiang Xiaojing He Weikun Hou Peng Xu Heng Du Rikard Holmdahl5 and Shemin Lu1 2 6 Abstract Introduction Toll-like receptors TLRs are likely to play crucial roles in the pathogenesis of rheumatoid arthritis RA . The aim of this study was to determine the key TLRs in synovium and explore their roles in the activation of fibroblast-like synoviocytes FLSs mediated by T cells in arthritis. Methods Pristane-induced arthritis PIA was established by subcutaneous injection with pristane at the base of the rat s tail. TLR expression in synovium from PIA rats was detected at different time points by performing realtime PCR. Polyinosinic polycytidylic acid poly I C was intra-articularly administrated to PIA rats and arthritis was monitored macroscopically and microscopically. Synovial TLR3 was detected by immunohistochemical staining. Rat FLSs were stimulated with pristane-primed T cells or pristane-primed T-cell conditioned medium. The intervention of TLR3 in FLSs was achieved by specific short-hairpin RNA shRNA or an antibody. The migration ability of FLSs was measured by using the scratch test and gene expression was detected by using real-time PCR. FLSs from RA patients were stimulated with various cytokines and TLR ligands and TLR3 expression was detected by performing real-time PCR. In addition with different concentrations of poly I C stimulation TLR3 expression of FLSs from RA patients and patients with osteoarthritis OA was compared. Results Synovium TLR3 displayed early and persistent overexpression in PIA rats. TLR3 was expressed in FLSs and local treatment with poly I C synergistically aggravated the arthritis. Rat FLSs co-cultured with pristane-primed T cells showed strengthened .

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