TAILIEUCHUNG - Báo cáo y học: " Effects of intratracheal administration of nuclear factor-kappaB decoy oligodeoxynucleotides on long-term "

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Effects of intratracheal administration of nuclear factor-kappaB decoy oligodeoxynucleotides on long-term. | Respiratory Research o BioMed Central Effects of intratracheal administration of nuclear factor-kappaB decoy oligodeoxynucleotides on long-term cigarette smoke-induced lung inflammation and pathology in mice Yu-Tao Li Bei He Yu-Zhu Wang and Jing Wang Address Department of Respiratory Medicine Peking University Third Hospital of Beijing PR China Email Yu-Tao Li - Bei He - puh3_hb@ Yu-Zhu Wang - bysyhuxike@ Jing Wang - zhouqingtaobysy@ Corresponding author Published 25 August 2009 Respiratory Research 2009 10 79 doi l465-992l-l0-79 Received 7 February 2009 Accepted 25 August 2009 This article is available from http content l0 l 79 2009 Li et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract To determine if nuclear factor-KB NF-kB activation may be a key factor in lung inflammation and respiratory dysfunction we investigated whether NF-kB can be blocked by intratracheal administration of NF-kB decoy oligodeoxynucleotides ODNs and whether decoy ODN-mediated NF-kB inhibition can prevent smoke-induced lung inflammation respiratory dysfunction and improve pathological alteration in the small airways and lung parenchyma in the long-term smoke-induced mouse model system. We also detected changes in transcriptional factors. In vivo the transfection efficiency of NF-kB decoy ODNs to alveolar macrophages in BALF was measured by fluorescein isothiocyanate FITC -labeled NF-kB decoy ODNs and flow cytometry post intratracheal ODN administration. Pulmonary function was measured by pressure sensors and pathological changes were assessed using histology and the pathological Mias software. NF-kB and activator protein l AP-l activity was detected by the

TỪ KHÓA LIÊN QUAN
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.