TAILIEUCHUNG - Báo cáo khoa học: Recent contributions of in vitro models to our understanding of hepatitis C virus life cycle

Hepatitis C virus is a human pathogen responsible for liver diseases includ-ing acute and chronic hepatitis, cirrhosis and hepatocellular carcinoma. Its high prevalence, the absence of a prophylactic vaccine and the poor effi-ciency of current therapies are huge medical problems. Since the discovery of the hepatitis C virus, our knowledge of its biology has been largely punctuated by the development of original models of research. | IFEBS Journal REVIEW ARTICLE Recent contributions of in vitro models to our understanding of hepatitis C virus life cycle Morgane Régeard Charlotte Lepere Maud Trotard Philippe Gripon and Jacques Le Seyec INSERM U522 IFR 140 Hopitalde Pontchaillou Rennes France Keywords assembly hepatitis C virus in vitro models infection replication Correspondence J. Le Seyec INSERM U522 Hopital Pontchaillou Avenue Henri Le Guilloux Rennes F-35033 France Fax 33 2 99 54 01 37 Tel 33 2 99 54 74 07 E-mail Received 14 June 2007 revised 25 July 2007 accepted 26 July 2007 doi Hepatitis C virus is a human pathogen responsible for liver diseases including acute and chronic hepatitis cirrhosis and hepatocellular carcinoma. Its high prevalence the absence of a prophylactic vaccine and the poor efficiency of current therapies are huge medical problems. Since the discovery of the hepatitis C virus our knowledge of its biology has been largely punctuated by the development of original models of research. At the end of the 1980s the chimpanzee model led to cloning of the viral genome and the definition of infectious molecular clones. In 1999 a breakthrough was achieved with the development of a robust in vitro replication model named replicon . This system allowed intensive research into replication mechanisms and drug discovery. Later in 2003 pseudotyped retroviruses harbouring surface proteins of hepatitis C virus were produced to specifically investigate the viral entry process. It was only in 2005 that infectious viruses were produced in vitro enabling intensive investigations into the entire life cycle of the hepatitis C virus. This review describes the different in vitro models developed to study hepatitis C virus their contribution to current knowledge of the virus biology and their future research applications. Introduction The hepatitis C virus HCV belongs to the Flaviviri-dae family and is the only member of the .

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