TAILIEUCHUNG - Báo cáo khoa học: Expression profiling reveals differences in metabolic gene expression between exercise-induced cardiac effects and maladaptive cardiac hypertrophy

While cardiac hypertrophy elicited by pathological stimuli eventually leads to cardiac dysfunction, exercise-induced hypertrophy does not. This sug-gests that a beneficial hypertrophic phenotype exists. In search of an under-lying molecular substrate we used microarray technology to identify cardiac gene expression in response to exercise. Rats exercised for seven weeks on a treadmill were characterized by invasive blood pressure mea-surements and echocardiography. | iFEBS Journal Expression profiling reveals differences in metabolic gene expression between exercise-induced cardiac effects and maladaptive cardiac hypertrophy Claes C. Str0m1 Mark Aplin1 Thorkil Ploug2 Tue E. H. Christoffersen1 Jozef Langfort3 Michael Viese2 Henrik Galbo2 Stig Hauns01 and S0ren P. Sheikh1 1 CHARC Copenhagen Heart Arrhythmia Research Center Department of Medicine B H S Rigshospitalet University of Copenhagen MedicalSchool Denmark 2 Copenhagen Muscle Research Centre Department of MedicalPhysiology Panum Institute University of Copenhagen Denmark 3 Laboratory of ExperimentalPharmacology Polish Academy of Science Warsaw Poland Keywords adaptive DNA microarray gene expression hypertrophy maladaptive Correspondence S. P. Sheikh Laboratory of Molecular Cardiology Department of Medicine B H S Rigshospitalet University of Copenhagen 20 Juliane Mariesvej DK-2100 Copenhagen Denmark Fax 45 3545 6500 Tel 45 3545 6730 E-mail sheikh@ Received 10 October 2004 revised 15 March 2005 accepted 22 March 2005 doi While cardiac hypertrophy elicited by pathological stimuli eventually leads to cardiac dysfunction exercise-induced hypertrophy does not. This suggests that a beneficial hypertrophic phenotype exists. In search of an underlying molecular substrate we used microarray technology to identify cardiac gene expression in response to exercise. Rats exercised for seven weeks on a treadmill were characterized by invasive blood pressure measurements and echocardiography. RNA was isolated from the left ventricle and analysed on DNA microarrays containing 8740 genes. Selected genes were analysed by quantitative PCR. The exercise program resulted in cardiac hypertrophy without impaired cardiac function. Principal component analysis identified an exercise-induced change in gene expression that was distinct from the program observed in maladaptive hypertrophy. Statistical analysis identified 267 upregulated genes and 62 .

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