TAILIEUCHUNG - Báo cáo khoa học: Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin

Mucins are large glycoproteins protecting mucosal surfaces throughout the body. Their expressions are tissue-specific, but in disease states such as cys-tic fibrosis, inflammation and cancer, this specificity can be disturbed. MUC5AC is normally expressed in the mucous cells of the epithelia lining the stomach and the trachea, where it constitutes a major component of the gastric and respiratory mucus. | ỊFEBS Journal Mapping of the 45M1 epitope to the C-terminal cysteine-rich part of the human MUC5AC mucin Martin E. Lidell1 Jacques Bara2 and Gunnar C. Hansson1 1 Department of MedicalBiochemistry Goteborg University Sweden 2 U-673 INSERM HopitalSaint-Antoine Paris France Keywords antibody 45M1 monoclonal MUC5AC mucin Correspondence G. C. Hansson Department of Medical Biochemistry Institute of Biomedicine Goteborg University Box 440 405 30 Gothenburg Sweden Fax 46 31 416108 Tel 46 31 7863488 E-mail Received 25 October 2007 accepted 28 November 2007 doi Mucins are large glycoproteins protecting mucosal surfaces throughout the body. Their expressions are tissue-specific but in disease states such as cystic fibrosis inflammation and cancer this specificity can be disturbed. MUC5AC is normally expressed in the mucous cells of the epithelia lining the stomach and the trachea where it constitutes a major component of the gastric and respiratory mucus. A number of mAbs have been raised against the gastric M1 antigen an early marker for colonic carcinogenesis. Several of these mAbs recognize epitopes present on MUC5AC suggesting that MUC5AC is the antigen. However some of the mAbs raised against the gastric M1 antigen are widely used as antibodies against MUC5AC despite the fact that their specificity for MUC5AC has not been clearly shown. In this study we have tested the reactivity of the latter antibodies against a recombinantly expressed C-terminal cysteine-rich part of human MUC5AC. We demonstrate for the first time that the widely used mAb 45M1 as well as 2-12M1 and 166M1 are true antibodies against MUC5AC with epitopes located in the C-terminal cysteine-rich part of the mucin. Mucins are large glycoproteins found on mucosal surfaces throughout the body. They are divided into membrane-bound and secreted mucins and the latter group can be further subdivided into gel-forming and nongel-forming mucins 1 . The .

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