TAILIEUCHUNG - Advances in Biomimetics Part 15

Tham khảo tài liệu 'advances in biomimetics part 15', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 482 Advances in Biomimetics An optimal scaffold for stem cell applications for vocal fold regeneration would be noninflammatory nonimmunogenic encourage adherence and viability of resident cells support appropriate cell-cell signaling biodegrade at an acceptable rate remain intact during investigator handling as well as be able to sustain vocal fold vibration. The scaffold materials listed previously have demonstrated some of these attributes in animal models but applications in conjunction with stem cell approaches is scant currently. There exists a great opportunity to advance vocal fold regeneration strategies by finding an optimal scaffold to deliver cells and growth factors. Growth factor delivery To date the delivery of only a few growth factors including epidermal growth factor EGF fibroblast growth factor FGF and hepatocyte growth factor HGF have been investigated within MSC-based therapies for vocal fold regeneration. All of this work has been completed in vitro. The effect of soluble signaling has been used to examine the differentiation potential of ASCs. A bilayered three dimensional construct was created in vitro by seeding ASCs within fibrin hydrogels and once gelation was complete additional ASCs were added directly on top. When EGF FGF and retinoic acid were added to the media surrounding these constructs it was found that EGF encouraged differentiation of ASCs into epithelial cells more efficiently than the other soluble signals Long et al. 2009 . The authors found that the cells on the top epithelial-like surface stained positive for E-cadherin and cytokeratin 8 epithelial phenotype markers. It was found that these cells differentiated along this lineage only when they had an air interface and exposure to EGF. Interestingly the authors hypothesized that mechanotransduction may have also played a role in differentiation as the cells were cultured on a matrix with similar stiffness to the lamina propria. The cells on the inside of the hydrogel .

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