TAILIEUCHUNG - báo cáo khoa học: "Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells | Wei et al. Journal of Experimental Clinical Cancer Research 2011 30 67 http content 30 1 67 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Recombinant immunotoxin anti-c-Met PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells 1 1 2 1 1 I- 7 z- - 2 1 Xu Wei Zhu Xiao Juan Feng Xiao Min Cai Nan Zhang Xiu Hua Feng Zheng Qing and Liu Zheng Abstract Background Our study aims to evaluate the anti-growth effects of recombinant immunotoxin IT anti-c-Met PE38KDEL on gastric cancer cells and its mechnisms. Methods Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay CCK . 3H -leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays. Results Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were and respectively when treated with 50 ng ml of IT. There were significant increase ofcaspase-3 activity at 24 hr of IT treatment 100 ng ml P in these gastric cancer cell lines. Conclusions IT anti-c-Met PE38KDEL has anti-growth effects on the gastric cancer cell lines in vitro and it provides an experimental basis for c-Met-targeted therapy towards in vivo testing. Introduction Gastric carcinoma GC is one of the most common and lethal malignant cancers 1 . Despite the improving surgical techniques and new chemotherapeutic treatment regimens the patient survival rate remains dismal 2 and effective alternative treatment approach is in vital need. GC has been shown to harbor multiple somatic mutations as well as over-expressions of oncoproteins. .

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