TAILIEUCHUNG - Báo cáo khoa học: The Pdx1 family is structurally and functionally conserved between Arabidopsis thaliana and Ginkgo biloba

Vitamin B6 is one of the most important compounds in living organisms, and its biosynthesis has only recently been understood. Because it is required for more than 100 biochemical reactions, lack of the vitamin is fatal. This is of special importance to mammals and humans, which cannot biosynthesize the vitamin and thus depend on its external uptake. Here we describe the cloning of a vitamin B6 biosynthetic gene GbPDX1from Ginkgo biloba. | ễFEBS Journal The Pdx1 family is structurally and functionally conserved between Arabidopsis thaliana and Ginkgo biloba Jan E. Leuendorf1 Andreas Genau2 Agnieszka Szewczyk3 Sutton Mooney4 Christel Drewke2 Eckhard Leistner2 and Hanjo Hellmann1 4 1 Angewandte Genetik Freie Universitat Berlin Germany 2 Istitut fur Pharmazeutische Biologie Rheinische Friedrich-Wilhelms-Universitat Bonn Germany 3 PharmaceuticalFaculty of the Collegium Medicum Jagiellonian University Krakow Poland 4 Schoolof BiologicalSciences Washington State University Pullman USA Keywords Ginkgo biloba ginkgotoxin PDX1 PDX2 vitamin B6 Correspondence H. Hellmann Schoolof BiologicalSciences Washington State University Pullman WA 99164-4236 USA Fax 1 509 335 3184 Tel 1 509 335 2762 E-mail hellmann@ Database Sequences from this article have been submitted to the GenBank database DDBJ EMBL data libraries under the accession numbers NP_181358 NP_188226 NP_195761 and AtPDX2 NP_568922 . GgPDX1 AAK18310 Received 15 October 2007 revised 4 December 2007 accepted 21 December 2007 doi Vitamin B6 is one of the most important compounds in living organisms and its biosynthesis has only recently been understood. Because it is required for more than 100 biochemical reactions lack of the vitamin is fatal. This is of special importance to mammals and humans which cannot biosynthesize the vitamin and thus depend on its external uptake. Here we describe the cloning of a vitamin B6 biosynthetic gene GbPDXl from Ginkgo biloba. The gene is expressed in seeds leaf and trunk tissue. Using yeast 2-hybrid and pull-down assays we show that the protein can interact with itself and with members of Arabidopsis thaliana AtPDX1 and At-PDX2 families. Furthermore we prove the function of GbPDX1 in vitamin B6 biosynthesis by complementation of an Arabidopsis mutant rsr4-1 at the phenotypical level and increasing vitamin B6 levels caused by ectopic GbPDX1 .

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