TAILIEUCHUNG - Báo cáo y học: "Transcriptome content and dynamics at single-nucleotide resolution"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Transcriptome content and dynamics at single-nucleotide resolution. | Minireview Transcriptome content and dynamics at single-nucleotide resolution Nicole Cloonan and Sean M Grimmond Address Institute for Molecular Bioscience University of Queensland 306 Carmody Road St Lucia 4072 Australia. Correspondence Sean M Grimmond. Email Published 18 September 2008 GenomeBiology 2008 9 234 doi gb-2008-9-9-234 The electronic version of this article is the complete one and can be found online at http 2008 9 9 234 2008 BioMed Central Ltd Abstract Massively parallel short-tag sequencing of cDNA libraries - RNAseq - is being used to study the dynamics and complexity of eukaryotic transcriptomes giving new biological insights into the active genome . With the advent of third-generation sequencing technologies -the so-called massively parallel sequencing technologies - it is now possible to generate tens of millions of short sequences each typically 25-50 nucleotides long in a single assay. This technology has enabled the recent RNA sequencing RNAseq via random cDNA libraries of the transcriptomes of yeasts Arabidopsis mouse embryonic stem ES cells and other mouse tissues and human cell lines. These experiments are helping to redefine the understanding of transcriptome content complexity and dynamics in these species. A recent study by Bahler and colleagues 1 in the fission yeast Schizosaccharomyces pombe in particular shows how the new RNAseq technology is ideally suited to revealing the changes that occur in transcriptional activity at different stages in the yeast life cycle and in response to changes in external conditions. Conceptually the RNAseq approach is very simple. Sequence reads are generated from random locations along each RNA by either sequencing sheared double-stranded cDNA libraries 1-4 strandless RNAseq or by sequencing directional cDNA libraries prepared using either adaptor-tagged random hexamers 5 or serial ligation of adaptors 6 to fragmented RNA populations stranded RNAseq . .

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