TAILIEUCHUNG - Báo cáo khoa học: Effect of oxidative stress and involvement of poly(ADP-ribose) polymerase (PARP) in Dictyostelium discoideum development

Dictyostelium discoideum, a unicellular eukaryote, exhibits multicellularity upon nutrient starvation and is a good model system for developmental studies, and for the study of various signal transduction pathways. Reac-tive oxygen species at low doses act as signaling molecules; | ỊFEBS Journal Effect of oxidative stress and involvement of poly ADP-ribose polymerase PARP in Dictyostelium discoideum development Jyotika Rajawat Iqbal Vohra Hina A. Mir Dhaval Gohel and Rasheedunnisa Begum Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara India Keywords benzamide development Dictyostelium discoideum oxidative stress PARP Correspondence R. Begum Department of Biochemistry Faculty of Science The Maharaja Sayajirao University of Baroda Vadodara-390002 India Fax 91 265 2795563 Tel 91 265 2795594 E-mail rasheedunnisab@ These authors contributed equally to this work Received 16 June 2007 revised 23 August 2007 accepted 3 September 2007 doi Dictyostelium discoideum a unicellular eukaryote exhibits multicellularity upon nutrient starvation and is a good model system for developmental studies and for the study of various signal transduction pathways. Reactive oxygen species at low doses act as signaling molecules however at high doses they are known to cause DNA damage that results in the activation of poly ADP-ribose polymerase PARP . We have earlier reported the high resistance of the unicellular stage of D. discoideum to oxidative stress and we now show the response of this organism to oxidative stress and the role of PARP during development. We used hydroxylamine HA to induce in situ generation of H2O2 and monitored the effect of benzamide a PARP inhibitor on oxidative stress-induced changes in D. discoideum development. Interestingly oxidative stress resulted in PARP activation within 5 min that was inhibited by benzamide. Oxidative stress-induced delay in developmental pattern was also partially restored by benzamide. We studied the long-term effects of PARP inhibition under oxidative stress and our results demonstrated that spores formed under HA stress exhibited significant delay in germination in comparison to benzamide-pretreated HA-stressed cells. .

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