TAILIEUCHUNG - Báo cáo y học: "Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells. | Li et al. Chinese Medicine 2011 6 19 http content 6 1 19 CHINESE MEDICINE RESEARCH Open Access Neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cells Xiao-Fan Li1 Cathy Nga-Ping Lui1 Zhi-Hong Jiang2 and Yung Kin-Lam Ken1 Abstract Background The present study investigates the effects of ginsenosides Rh1 and Rg2 against 6-hydroxydopamine 6-OHDA a neurotoxin on SH-SY5Y cells and PC-12 cells. The effects of these two ginsenosides on neuronal differentiation are also examined. Methods LDH assay was used to measure cell viability after exposure to 6-OHDA and ginsenosides. Neuronal differentiation was evaluated by changes in cell morphology and density of neurite outgrowths. Western blotting was used to determine the ginsenosides effects on activation of extracellular signal-regulated protein kinases ERKs . Results Rh1 and Rg2 attenuated 6-OHDA toxicity in SH-SY5Y cells and induced neurite outgrowths in PC-12 cells. 6-OHDA-induced ERK phosphorylation was decreased by Rh1 and Rg2. 20 R -form and 20 S -form of the ginsenosides exerted similar effects in inducing neurite outgrowths in PC-12 cells. Conclusion The present study demonstrates neuroprotective effects of ginsenosides Rh1 and Rg2 on neuronal cell lines. These results suggest potential Chinese medicine treatment for neurodegenerative disorders eg Parkinson s disease . Background Parkinson s disease PD is a common motor system disorder characterized clinically by rigidity resting tremor and slow movements 1 . It is associated with a progressive loss of dopaminergic neurons within the substantia nigra and depletion of dopamine in the striatal region 2 3 . Dopamine DA is a catecholamine neurotransmitter in the brain produced mainly in the substantia nigra and the ventral tegmental area. Six-hydroxydopamine 6-OHDA is a hydroxylated analogue of DA. Metabolism of dopamine leads to the generation 6-OHDA 4 5 which exerts specific neurotoxicity on catecholaminergic neurons by a selective .

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