TAILIEUCHUNG - Value of flowcytometry and PCR-SSP techniques in detection of HLA-B27

In the present study, detected HLA-B27 in blood samples of patients suspected to have AS, by both flowcytometry-based and PCR-SSP-based assays, in order to assess the value of each method in HLA-B27 detection. | Journal of military pharmaco-medicine 7-2013 VALUE OF FLOWCYTOMETRY AND PCR-SSP TECHNIQUES IN DETECTION OF HLA-B27 Le Xuan Hai*; Nguyen Dang Dung** Summary HLA-B27 has been demonstrated to be strongly associated with ankylosing spondylitis (AS), a chronic inflammatory disease affecting the axial muscoskeletal system. Routine clinical tests detecting HLA-B27 include cytotoxicity, immunofluorescent, and flowcytometry-based assays, among which the later showed flowcytometry assays are the best advantages of specificity and rapidity in performance. In recent years, however, application of PCR-based DNA typing resulted in a more reliable assay for HLA-B27 detection. In the present study, we detected HLA-B27 in blood samples of patients suspected to have AS, by both flowcytometry-based and PCR-SSP-based assays, in order to assess the value of each method in HLA-B27 detection. The results showed that: - Flowcytometry-based assay can detect cell surface HLA-B27 expression when positive and negative events got a clear cut over the cut-off level (the real positive and negative results). - PCR-SSP-based assay is of much help in detecting HLA-B27 encoding gene when flowcytometry-based assay was unable to determine the expression of HLA-B27 (the undetermined results). * Key words: HLA-B27; Flowcytometry; Polymerase chain reaction (PCR); Ankylosing spodylitis. INTRODUCTION HLA-B27 is a protein encoded by an allele of locus HLA-B, one of three loci within the human class I major histocompatibility complex (MHC). It is well demonstrated that the expression of HLA-B27 strongly associated with ankylosing spondylitis (AS), a chronic inflammatory disease affecting the axial muscoskeletal system in human. According to statistical data, about 1% of people with HLA-B27 positive would develop AS; however, 90 95% of patients with AS had HLA-B27 positive expression, while in patients without the disease, this incidence was 5 - 8% only. The role of HLA-B27 in pathogenesis of AS has .

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