TAILIEUCHUNG - Effects of umbilical cord exctracts on proliferation and gene expression of cultured human skin fibroblast

This study tested the effects of newborn umbilical cord extracts on the proliferation and synthesis of collagen type 1, fibronectin and metalloproteinase 1 (MMP-1) of human skin fibroblast. | Journal of military pharmaco-medicine 7-2013 EFFECTS OF UMBILICAL CORD EXCTRACTS ON PROLIFERATION AND GENE EXPRESSION OF CULTURED HUMAN SKIN fIBROBLAST Pham Van Phuc*; Dang Thi Tung Loan*; Phan Kim Ngoc* Dinh Thanh Uyen**; Le Van Dong*** summary Wrinkles are consequences of skin aging. This process is characterized with collagen breakdown and shortage in skin structure. This study tested the effects of newborn umbilical cord extracts on the proliferation and synthesis of collagen type 1, fibronectin and metalloproteinase 1 (MMP-1) of human skin fibroblast. 25 formulas made of 4 types of extracts included: extracellular extract of cord tissue; intracellular extract of cord cells; mixtures of these two extracts; intracellular extract of umbilical cord stem cells, were supplemented to culture media of human fibroblast. Cell proliferation was evaluated by MTT assay; gene expression was analyzed by Real-time RT-PCR technique. The results showed that supplementation of of formula 3-5 containing a combination of both extra- and intra-cellular extracts of umbilical cord led to the highest cell proliferation and also stimulated the expression of collagen type 1 while inhibited the expression of MMP-1 on fibroblast. These data suggested that umbilical cord extract would be a potential source of material for wrinkle reduction skin care product. * Key words: Skin aging; Wrinkle; Stem cel; Umbilical cord extract. INTRODUCTION Aging is the issue that is getting more and more attentions because of its social impacts. Skin aging is known to be caused mainly by exposure to light since then it is being referred as photo-aging, in which sunlight UV is main cause. UV ray activates signal transductions via protein kinases in cells located through dermis leading to the over expression of genes related to proteolysis of proteins in extracellular matrix such as metalloproteinase (MMP) 1 (collagenase), MMP-3 (stromelysis 1), and MMP-9 (gelatinase) [2, 5]. MMP-1 cuts collagen type I,

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