TAILIEUCHUNG - Study on inhibitory effect of Ngoc Linh ginseng stem cell extract on collagen degradation in fibroblasts by 2D-PAGE

The ability of Ngoclinh ginseng (NLG) stem cell extract in controlling the skin aging process in human fibroblasts was investigated. To evaluate the cosmeceutical effects of the extracts on the aging process, MTT assays were conducted with various concentrations of NLG stem cells. No extracts showed any cytotoxic activities on fibroblasts at concentrations of up to 10 mg/ml. | Journal of military pharmaco-medicine 7-2013 STUDY ON INHIBITORY EFFECT OF NGOC LINH GINSENG STEM CELL EXTRACT ON COLLAGEN DEGRADATION IN FIBROBLASTS BY 2D-PAGE Nguyen Van Thinh*; Vu Tuan Anh*; Nguyen Huu Tuan Dung** Pham Xuan Phong***; Nguyen Van Long* * Vietnam Military Medical University ** University of Dalat ***Military Institute of Traditional Medicine SUMMARY The ability of Ngoclinh ginseng (NLG) stem cell extract in controlling the skin aging process in human fibroblasts was investigated. To evaluate the cosmeceutical effects of the extracts on the aging process, MTT assays were conducted with various concentrations of NLG stem cells. No extracts showed any cytotoxic activities on fibroblasts at concentrations of up to 10 mg/ml. An effect on the degradation of collagen matrix was observed when the dose concentration was mg/ml. Proteome profiling analysis showed that the extract promoted the expression of proteins related to collagen production and suggested the skin aging effect of the extract. The extract could be considered as an attractive, anti-wrinkle ingredient for cosmetic applications. * Key words: Ngoclinh ginseng; Collagen; 2-D PAGE; fibroblasts. INTRODUCTION The mechanism of collagen degradation is primarily attributable to the activation of growth factor receptors on the surface of fibroblasts and keratinocytes caused by UV irradiation, resulting in signal transduction through a protein kinase cascade and subsequent activation of AP-1 in the nucleus. The signal transduction could also be strongly up-regulated by ROS induced by UV irradiation. This then stimulates MMP production in both dermis and epidermis and leads to the degradation of collagen and elastic fibers. Ngoclinh ginseng (Vietnamese ginseng, Panax vietnamensis Ha et Grushv. Araliaceae) is a wild Panax species that has been used as a herbal medicine in Central Vietnam. In recent years, NLG has been cultured in bioreactors for production of ginseng saponin and ginsenosides for .

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