TAILIEUCHUNG - Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities

GlutathioneS-transferases (EC ) (GSTs), are a family of multifunctional enzymes present in all living organisms whose main function is the detoxification of electrophilic compounds. GSTs are considered the most prominent detoxifying class II enzymes in helminths. We describehere the characterizationof novel dehydroascorbate reductase and thiol transferase activities that reside in the human parasite Schistosoma mansoni GSTx. | Eur. J. Biochem. 269 5512-5521 2002 FEBS 2002 doi Characterization of an omega-class glutathione S-transferase from Schistosomamansoniwith glutaredoxin-like dehydroascorbate reductase and thiol transferase activities Javier Girardini1 Alejandro Amirante2 t Khalid Zemzoumi1 and Esteban Serra1 1Instituto de Biologia Molecular y Celular de Rosario IBR-CONICET Facultad de Ciencias Bioquimicas y Farmaceuticas UNR and 2Facultad de Odontologia UNR Rorario Argentina Glutathione S-transferases EC GSTs are a family of multifunctional enzymes present in all living organisms whose main function is the detoxification of electrophilic compounds. GSTs are considered the most prominent detoxifying class II enzymes in helminths. We describe here the characterization of novel dehydroascorbate reductase and thiol transferase activities that reside in the human parasite Schistosoma mansoni GSTx. Protein sequence analysis of this parasite product showed lower identity to known GSTs. However phylogenic analysis placed SmGSTx among the recently described omega class GSTs GSTO1-1 . We reporthere that SmGSTO protein is a 28-kDa polypeptide detected in all life stages of the parasite being highly expressed in adult worms. Like other omega class GSTs SmGSTO showed very low activity toward classical GSTs substrates as 1-chloro-2 4-dinitrobenzene and no binding affinity to glutathione-agarose matrix but showed some biochemical characteristics related with thio-redoxins glutaredoxins. Interestingly SmGSTO was able to bind S-hexyl glutathione matrix and displayed significant glutathione-dependent dehydroascorbate reductase and thiol transferase enzymatic activities. Keywords glutathione S-transferase dehydroascorbate reductase thiol transferase Schistosoma. Glutathione S-transferases GSTs EC constitute a family of multifunctional enzymes that mainly catalyze the nucleophilic attack of reduced glutathione GSH to a wide variety of electrophilic .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine