TAILIEUCHUNG - Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines

Three pairs of parental (q + ) and established mitochondrial DNAdepleted (q 0 ) cells, derived frombone, lungandmuscle were used to verify the influence of the nuclear background and the lack of efficient mitochondrial respiratory chain on antioxidant defences and homeostasis of intracellular reactive oxygen species (ROS). Mitochondrial DNA deple-tion significantly lowered glutathione reductase activity, glutathione (GSH) content, and consistently altered the GSH 2 : oxidized glutathione ratio in all of theq 0 cell lines, albeit todiffering extents, indicating themost oxidized redox state inboneq 0 cells | Eur. J. Biochem. 271 3646-3656 2004 FEBS 2004 doi Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines Lodovica Vergani1. Maura Floreani2 Aaron Russell3 Mara Ceccon1 Eleonora Napoli4 Anna Cabrelle5 Lucia Valente2 Federica Bragantini1 Bertrand Leger3 and Federica Dabbeni-Sala2 1 Dipartimento di Scienze Neurologiche and 2Dipartimento di Farmacologia e Anestesiologia Universita di Padova Padova Italy Clinique Romande de Readaptation SUVA Care Sion Switzerland Scientific Institute Conegliano Research Centre Conegliano Italy 5Dipartimento di Medicina Clinica Universita di Padova c o Istituto Veneto di Medicina Molecolare Padova Italy Three pairs of parental p and established mitochondrial DNA depleted p0 cells derived from bone lung and muscle were used to verify the influence of the nuclear background and the lack of efficient mitochondrial respiratory chain on antioxidant defences and homeostasis of intracellular reactive oxygen species ROS . Mitochondrial DNA depletion significantly lowered glutathione reductase activity glutathione GSH content and consistently altered the GSH2 oxidized glutathione ratio in all of the p0 cell lines albeit to differing extents indicating the most oxidized redox state in bone p0 cells. Activity as well as gene expression and protein content of superoxide dismutase showed a decrease in bone and muscle p0 cell lines but not in lung p0 cells. GSH peroxidase activity was four times higher in all three p0 cell lines in comparison to the parental p suggesting that this may be a necessary adaptation for survival without a functional respiratory chain. Taken together these data suggest that the lack of respiratory chain prompts the cells to reduce their need for antioxidant defences in a tissue-specific manner exposing them to a major risk of oxidative injury. In fact bone-derived p0 cells displayed the highest steady-state level of .

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• oxidation
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• analysis of cytoplasmic
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