TAILIEUCHUNG - Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity

Retinol dehydrogenase 13 (RDH13) is a recently identified short-chain dehydrogenase⁄reductase related to microsomal retinoid oxidoreductase RDH11. In this study, we examined the distribution of RDH13 in human tissues, determined its subcellular localization and characterized the sub-strate and cofactor specificity of purified RDH13 in order to better understand its properties. | ễFEBS Journal Human retinol dehydrogenase 13 RDH13 is a mitochondrial short-chain dehydrogenase reductase with a retinaldehyde reductase activity Olga V. Belyaeva Olga V. Korkina Anton V. Stetsenko and Natalia Y. Kedishvili Department of Biochemistry and Molecular Genetics Schools of Medicine and Dentistry University of Alabama at Birmingham AL USA Keywords dehydrogenase mitochondria reductase retinaldehyde retinol Correspondence N. Y. Kedishvili Division of Biochemistry and Molecular Genetics Schools of Medicine and Dentistry University of Alabama at Birmingham 720 20th Street South 440B KaulGenetics Building Birmingham AL 35294 USA Fax 205 934 0758 Tel 205 996 4023 E-mail nkedishvili@ Present address Department of Biochemistry Tufts University Schoolof Medicine Boston MA USA TAbbott Vascular Abbott Park IL USA Received 12 September 2007 revised 31 October 2007 accepted 7 November 2007 doi Retinol dehydrogenase 13 RDH13 is a recently identified short-chain dehydrogenase reductase related to microsomal retinoid oxidoreductase RDH11. In this study we examined the distribution of RDH13 in human tissues determined its subcellular localization and characterized the substrate and cofactor specificity of purified RDH13 in order to better understand its properties. The results of this study demonstrate that RDH13 exhibits a wide tissue distribution and by contrast with other members of the RDH11-like group of short-chain dehydrogenases reduc-tases is a mitochondrial rather than a microsomal protein. Protease protection assays suggest that RDH13 is localized on the outer side of the inner mitochondrial membrane. Kinetic analysis of the purified protein shows that RDH13 is catalytically active and recognizes retinoids as substrates. Similar to the microsomal RDHs RDH11 RDH12 and RDH14 RDH13 exhibits a much lower Km value for NADPH than for NADH and has a greater catalytic efficiency in the reductive than in the oxidative direction. .

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