TAILIEUCHUNG - Retrovirology Research BioMed Central Open Access Extracellular ATP reduces HIV-1 transfer from

Retrovirology Research BioMed Central Open Access Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes Corinne Barat, Caroline Gilbert, Michael Imbeault and Michel J Tremblay* Address: Laboratoire d'Immuno-Rétrovirologie Humaine, Centre de Recherche en Infectiologie, RC709, 2705 Boul. Laurier, Québec (QC), G1V 4G2, Canada Email: Corinne Barat - ; Caroline Gilbert - ; Michael Imbeault - ; Michel J Tremblay* - * Corresponding author Published: 28 March 2008 Retrovirology 2008, 5:30 doi: Received: 26 October 2007 Accepted: 28 March 2008 This article is available from: © 2008 Barat et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms. | Retrovirology BioMed Central Research Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4 T lymphocytes Corinne Barat Caroline Gilbert Michael Imbeault and Michel J Tremblay Open Access Address Laboratoire d Immuno-Rétrovirologie Humaine Centre de Recherche en Infectiologie RC709 2705 Boul. Laurier Québec QC G1V 4G2 Canada Email Corinne Barat - Caroline Gilbert - Michael Imbeault - Michel J Tremblay - Corresponding author Published 28 March 2008 Retrovirology 2008 5 30 doi 1742-4690-5-30 Received 26 October 2007 Accepted 28 March 2008 This article is available from http content 5 1 30 2008 Barat et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Dendritic cells DCs are considered as key mediators of the early events in human immunodeficiency virus type 1 HIV-1 infection at mucosal sites. Previous studies have shown that surface-bound virions and or internalized viruses found in endocytic vacuoles of DCs are efficiently transferred to CD4 T cells. Extracellular adenosine triphosphate ATP either secreted or released from necrotic cells induces a distorted maturation of DCs transiently increases their endocytic capacity and affects their migratory capacity. Knowing that high extracellular ATP concentrations are present in situations of tissue injury and inflammation we investigated the effect of ATP on HIV-1 transmission from DCs to CD4 T lymphocytes. Results In this study we show that extracellular ATP reduces HIV-1 transfer from immature monocyte-derived DCs iDCs to autologous CD4 T cells. This observed decrease in .

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