TAILIEUCHUNG - Báo cáo y học: "High systemic levels of interleukin-10, interleukin22 and C-reactive protein in Indian patients are associated with low in vitro replication of HIV-1 subtype C viruses"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Retrovirology cung cấp cho các bạn kiến thức về ngành y đề tài: "High systemic levels of interleukin-10, interleukin22 and C-reactive protein in Indian patients are associated with low in vitro replication of HIV-1 subtype C viruses. | Arias et al. Retrovirology 2010 7 15 http content 7 1 15 RETR0VIR0L0GY RESEARCH Open Access High systemic levels of interleukin-10 interleukin-22 and C-reactive protein in Indian patients are associated with low in vitro replication of HIV-1 subtype C viruses Juan F Arias1 2 Reiko Nishihara3 Manju Bala1 4 Kazuyoshi Ikuta1 Abstract Background HIV-1 subtype C HIV-1C accounts for almost 50 of all HIV-1 infections worldwide and predominates in countries with the highest case-loads globally. Functional studies suggest that HIV-1C is unique in its biological properties and there are contradicting reports about its replicative characteristics. The present study was conducted to evaluate whether the host cytokine environment modulates the in vitro replication capacity of HIV-1C viruses. Methods A small subset of HIV-1C isolates showing efficient replication in peripheral blood mononuclear cells PBMC is described and the association of in vitro replication capacity with disease progression markers and the host cytokine response was evaluated. Viruses were isolated from patient samples and the corresponding in vitro growth kinetics were determined by monitoring for p24 production. Genotype phenotype and co-receptor usage were determined for all isolates while clinical category CD4 cell counts and viral loads were recorded for all patients. Plasmatic concentrations of cytokines and acute-phase response and microbial translocation markers were determined and the effect of cytokine treatment on in vitro replication rates was also measured. Results We identified a small number of viral isolates showing high in vitro replication capacity in healthy-donor PBMC. HIV-1C usage of CXCR4 co-receptor was rare therefore it did not account for the differences in replication potential observed. There was also no correlation between the in vitro replication capacity of HIV-1C isolates and patients disease status. Efficient virus growth was significantly associated with

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