TAILIEUCHUNG - Báo cáo khoa học: The effect of hfq on global gene expression and virulence in Neisseria gonorrhoeae

Hfq is an RNA chaperone that functions as a pleiotropic regulator for RNA metabolism in bacteria. In several pathogenic bacteria, Hfq contrib-utes indirectly to virulence by binding to riboregulators that modulate the stability or translation efficiency of RNA transcripts. To characterize the role of Hfq in the pathogenicity ofNeisseria gonorrhoeae, we generated an N. gonorrhoeae hfqmutant. | ỊFEBS Journal The effect of hfq on global gene expression and virulence in Neisseria gonorrhoeae an 1I z t I o DIt ir h1 D zt t zt Ixlxrt 11nIsa 1 QrỈZ n z-v q hoi 1 F IIxzt 7icl a1 Ian Potor Rnnttr hnr1 Ivianueid Dietrich neuekka Iviuiiike Iviaiiuii uuLiouiiaiu Like Zioka Jan reier Doeiiuner Hans Moiienkopf2 and Alexandra Friedrich1 f 1 Max Planck Institute for Infection Biology Department of Molecular Biology Berlin Germany 2 Max Planck Institute for Infection Biology Microarray Core Facility Berlin Germany Keywords gene expression Hfq Neisseria gonorrhoeae RNA chaperone virulence Correspondence M. Dietrich Max Planck Institute for Infection Biology Department of Molecular Biology Chariteplatz 1 D-10117 Berlin Germany Fax 49 30 28460401 Tel 49 30 28460438 E-mail mdietrich@ f Present address FederalInstitute for Risk Assessment Diedersdorfer Weg 1 D-12277 Berlin Germany These authors contributed equally to this work Received 15 October 2008 revised 23 June 2009 accepted 23 July 2009 doi Hfq is an RNA chaperone that functions as a pleiotropic regulator for RNA metabolism in bacteria. In several pathogenic bacteria Hfq contributes indirectly to virulence by binding to riboregulators that modulate the stability or translation efficiency of RNA transcripts. To characterize the role of Hfq in the pathogenicity of Neisseria gonorrhoeae we generated an N. gonorrhoeae hfq mutant. Infectivity and global changes in gene expression caused by the hfq mutation in N. gonorrhoeae strain MS11 were analyzed. Transcriptional analysis using a custom-made N. gonorrhoeae microarray revealed that 369 ORFs were differentially regulated in the hfq mutant MS11hfq in comparison with the wild-type strain 202 were upreg-ulated and 167 were downregulated . The loss-of-function mutation in hfq led to pleiotropic phenotypic effects including an altered bacterial growth rate and reduced adherence to epithelial cells. Twitching motility and .

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