TAILIEUCHUNG - Báo cáo khoa học: Hypoxia induces erythroid-specific 5-aminolevulinate synthase expression in human erythroid cells through transforming growth factor-b signaling

Novel biotin-binding proteins, referred to herein as tamavidin 1 and tamavidin 2, were found in a basidiomycete fungus, Pleurotus cornucopiae, known as the Tamogitake mushroom. These are the first avidin-like proteins to be discovered in organisms other than birds and bacteria. | Hypoxia induces erythroid-specific 5-aminolevulinate synthase expression in human erythroid cells through transforming growth factor-b signaling Kiriko Kaneko1 Kazumichi Furuyama1 Hiroyuki Aburatani2 and Shigeki Shibahara1 1 Department of Molecular Biology and Applied Physiology Tohoku University Schoolof Medicine Miyagi Japan 2 Genome Science Division Research Center for Advanced Science and Technology University of Tokyo Japan Keywords erythroid-specific 5-aminolevulinate synthase hemoglobin hypoxia mitoferrin transforming growth factor-b Correspondence K. Furuyama Department of Molecular Biology and Applied Physiology Tohoku University Schoolof Medicine 2-1 Seiryo-machi Aoba-ku Sendai Miyagi 980-8575 Japan Fax 81 22 717 8118 Tel 81 22 717 8115 E-mail k-furuya@ Received 29 August 2008 revised 21 December 2008 accepted 24 December 2008 doi Hypoxia induces expansion of erythroid precursor cells through erythropoietin production. However it has also been suggested that hypoxia could enhance hemoglobin production in erythroid cells directly. To identify the molecules that are involved in hemoglobin production under hypoxia we examined the expression profile of mRNAs in YN-1 human erythroleukemia cells under hypoxia. DNA array analysis revealed that the expression of transforming growth factor TGF -b1 and mitoferrin which is a mitochondrial iron transporter was induced after 6 h under hypoxia in YN-1 cells whereas the increased expression of erythroid-specific 5-aminolevulinate synthase ALAS2 and y-globin mRNAs was observed after 48 h. Further analysis revealed that hypoxia enhanced the accumulation of TGF-b1 in the culture medium of cells of the YN-1-0-A line which was a clonal variant of YN-1 and could be maintained in serum-free medium. Moreover exogenous TGF-b1 induced hemoglobini-zation and the expression of ALAS2 mRNA in YN-1-0-A cells but not of y-globin and mitoferrin mRNAs. Importantly a specific .

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