TAILIEUCHUNG - báo cáo khoa học: " Potent cytotoxic effects of Calomeria amaranthoides on ovarian cancers"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Potent cytotoxic effects of Calomeria amaranthoides on ovarian cancers | van Haaften et al. Journal of Experimental Clinical Cancer Research 2011 30 29 http content 30 1 29 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Potent cytotoxic effects of Calomeria amaranthoides on ovarian cancers rr ilinci I r Pil il o2 Aril l l A oorhoim3 Dl I Qmit 4 Dm K IK I I l rH5 Pirr ii t 7 Pi rrmi iHi6 CdlUlllie vdn Hddlien Colin C Duke AriJ M weenieim Nicu PM Jinn Pdul MM vdn HddIU Firouz Ddnoudi Baptist JMZ Trimbos 1 Abstract Background Ovdridn cdncer remdins the ledding cduse of dedth from gyndecologicdl mdligndncy. More thdn 60 of the pdtients dre presenting the disedse in stdge III or IV. In spite of combindtion of chemotherapy dnd surgery the prognosis stdys poor for therapy regimen. Methods The ledves of d pldnt endemic to Austrdlid Calomeria amaranthoides were extracted dnd then frdctiondted by column chromdtogrdphy. In vitro cytotoxicity tests were performed with fractions of the pldnt extrdct dnd ldter with dn isoldted compound on ovdridn cdncer cell lines ds well ds normdl fibrobldsts dt concentrations of 1-100 gg mL crude extract dnd 1-10 gg mL compound . Cytotoxicity wds medsured dfter 24 48 dnd 72 hours by using d non-fluorescent substrate Aldmdr blue. In vivo cytotoxicity wds tested on dscites developed in the dbdomen of nude mice dfter inoculdtion with humdn OVCAR3 cells intrdperitonedlly. The rdte of chdnge in dbdomen size for the mice wds determined by linedr regression dnd stdtisticdlly evdludted for significdnce by the unpdired t test. Results Two compounds were isoldted by chromdtogrdphic frdctiondtion dnd identified by 1H-NMR 13C-NMR dnd mdss spectrometry dndlyses EPD dn a-methylene sesquiterpene ldctone of the eremophildnolide subtype dnd EPA dn a-methylene cdrboxylic dcid. Cytotoxicity of EPD for normdl fibrobldsts dt dll time points IC50 wds gredter thdn 10 gg mL whereds for OVCAR3 cells dt 48 hours IC50 wds gg mL 95 confidence intervdl to gg mL . Both the crude pldnt extract ds

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