TAILIEUCHUNG - Báo cáo y học: " Involvement of mTOR signaling in sphingosylphosphorylcholine-induced hypopigmentation effects"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Involvement of mTOR signaling in sphingosylphosphorylcholine-induced hypopigmentation effects. | Jeong et al. Journal of Biomedical Science 2011 18 55 http content 18 1 55 The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Involvement of mTOR signaling in sphingosylphosphorylcholine-induced hypopigmentation effects 1111 1 2 Hyo-Soon Jeong Seung Hoon Lee Hye-Young Yun Kwang Jin Baek Nyoun Soo Kwon Kyoung-Chan Park and Dong-Seok Kim1 Abstract Background Sphingosylphosphorylcholine SPC acts as a potent lipid mediator and signaling molecule in various cell types. In the present study we investigated the effects of SPC on melanogenesis and SPC-modulated signaling pathways related to melanin synthesis. Methods Melanin production was measured in Mel-Ab cells. A luciferase assay was used to detect transcriptional activity of the MITF promoter. Western blot analysis was performed to examine SPC-induced signaling pathways. Results SPC produced significant hypopigmentation effects in a dose-dependent manner. It was found that SPC induced not only activation of Akt but also stimulation of mTOR a downstream mediator of the Akt signaling pathway. Moreover SPC decreased the levels of LC3 II which is known to be regulated by mTOR. Treatment with the mTOR inhibitor rapamycin eliminated decreases in melanin and LC3 II levels by SPC. Furthermore we found that the Akt inhibitor LY294002 restored SPC-mediated downregulation of LC3 II and inhibited the activation of mTOR by SPC. Conclusions Our data suggest that the mTOR signaling pathway is involved in SPC-modulated melanin synthesis. Keywords Akt LC3 II Melanocytes mTOR sphingosylphosphorylcholine Background Melanin a pigment found in hair eyes and skin is produced by melanocytes and its synthesis is promoted by various stimulators such as UV irradiation hormones and cytokines 1-3 . At least 3 enzymes are required for melanin synthesis. Tyrosinase catalyses the first 2 ratelimiting steps of .

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