TAILIEUCHUNG - Báo cáo sinh học: "Robust and specific inhibition of microRNAs in Caenorhabditis elegans"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Robust and specific inhibition of microRNAs in Caenorhabditis elegans. | Kundu and Slack Journal of Biology 2010 9 20 http content 9 3 20 Journal of Biology MINIREVIEW L__ Robust and specific inhibition of microRNAs in Caenorhabditis elegans Samrat T Kundu and Frank J Slack See research article http content 1 1 9 Abstract MicroRNAs miRNAs are small non-coding RNAs that regulate the expression of numerous target genes. Yet while hundreds of miRNAs have been identified little is known about their functions. In a recent report published in Silence Zheng and colleagues demonstrate a technique for robust and specific knockdown of miRNA expression in Caenorhabditis elegans using modified antisense oligonucleotides which could be utilized as a powerful tool for the study of regulation and function of miRNAs in vivo. MicroRNAs miRNAs are approximately 22-nucleotide-long non-coding RNA molecules that were first discovered in Caenorhabditis elegans but are now known to be expressed in all metazoans. miRNA genes are present singly or in clusters often with their individual promoters and regulatory elements while some miRNA genes are also present within the introns of other protein coding genes. miRNAs are transcribed as long primary transcripts and are processed first in the nucleus by the microprocessor DRSH-1 DROSHA and then in the cytoplasm by the DCR-1 DICER complex to generate the active 21 to 23-nucleotide RNA molecules. These are recruited by the RNA-induced silencing complex RISC and guided to the 3 untranslated regions of target messenger RNAs mRNAs with complete or incomplete sequence complementarity resulting in either degradation of the target mRNA or inhibition of its translation. A single miRNA can target multiple mRNAs and this ability to control the expression of numerous genes makes miRNAs pivotal in regulating various life Correspondence Department of Molecular Cellular and Development Biology Yale University New Haven CT 06520 USA 2 BioMed Central 2010 BioMed Central

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