TAILIEUCHUNG - analysis of genes and genomes phần 8

Single-bước gen knock-outs trong men. Việc chuyển đổi men với một đoạn DNA tuyến tính có chứa một gen có thể lựa chọn (URA3 hình thức một phần của con đường sinh tổng hợp uracil) hai bên kết quả trình tự DNA chèn của nó tại khu vực tương đồng của bộ gen (được chỉ định bởi các đường đứt nét). | 328 POST-GENOME ANALYSIS 10 PCR product URA3 Genomic DNA Í. s _ Í s Gene 1 Ura URA3 Figure . Single-step gene knock-outs in yeast. The transformation of yeast with a linear DNA fragment containing a selectable gene URA3 forms part of the uracil biosynthetic pathway flanked by genomic DNA sequences results in its insertion at homologous regions of the genome indicated by the dashed lines . The gene between the homologous sequences is eliminated and replaced by the selectable gene cassette contains the KANR ORF of the E. coli Tn903transposon fused to transcriptional and translational control sequences of the TEF gene of the filamentous fungus Ashbya gossypii. The insertion of the cassette into the yeast genome permits efficient selection of transformants resistant to the antibiotic geneticin G418 . The disruption of a gene is likely to lead to one of the following phenotypes. Lethal. The deleted cell is unable to grow. The gene is essential for some part of the growth process. Essential genes in yeast can be distinguished by the way in which they segregate during sporulation. Wild type. There is no detectable difference between the mutant and wildtype cells. If no differences can be detected under a variety of conditions it may suggest that another gene is able to compensate for the loss or that particular conditions have not been found where the loss will show as a phenotypic change. Growth difference. The deleted strain may grow slower or faster than the wild-type parent under particular conditions. A battery of different growth tests each under a variety of conditions may then be used to identify potential roles of the protein encoded by the gene. The analysis of yeast disruption mutants showed that almost 20 per cent of yeast genes within the genome were essential for growth on glucose-rich media and in other screens 15 per cent of the disruptions had an effect on overall cell ANTISENSE AND RNA INTERFERENCE RNAi 329 size or morphology Giaever et al. 2002

• Sinh học
• Introduction to Thermal Analysis Methods
• Thermal Analysis used
• Thermal Analysis
• Thermal Gravimetric Analysis
• Differential Thermal Analysis
• Differential Scanning Calorimetry
• Chromatographic analysis
• Environmental analysis
• Food toxicants analysis
• Heterocyclic amine mutagens carcinogens
• Analysis of nitrosamines
• Intraocular pressure
• Meta analysis
• Meta analysis and trial sequential analysis
• Trial sequential analysis
• Increased intraocular pressure
• Tensile properties of cooked meat sausages
• Physico chemical analysis
• Textural analysis
• Statistical analysis
• Financial statement analysis
• Analyzing operating activities
• Cash flow analysis
• Profitability analysis
• Prospective analysis
• Credit analysis
• Trace Element Analysis
• Food
• Preparation Techniques
• Analytical Chemistry
• chemical analysis
• the overall chemical analysis
• chemical analysis of materials
• Chemometric Techniques
• Quantitative Analysis
• VITAMINS IN FOODS Analysis
• Bioavailability
• and Stability
• FOOD PROTEIN ANALYSIS Quantitative Effects on Processing
• how to use excel in analytical chemistry and in general scientific data analysis
• Multimodal discourse analysis
• Multimodal discourse
• Discourse analysis
• Analysis the picture
• Interpersonal meaning
• Financial statement
• Lecture Financial statement analysis
• Business analysis
• Financial statement analysis techniques
• Efficient market
• Structural analysis
• Basic principles of structural analysis
• The analysis of statically determinate
• Indeterminate beams
• Rigid frames
• The matrix analysis of structures
• Applied multivariate statistical analysis
• Multivariate analysis
• Multivariate linear regression models
• Canonical correlation analysis
• Distance methods
• Principal component analysis
• Genetic diversity
• Principal component analysis approach
• Genetic diversity analysis
• Brinjal (Solanum melongena L
• Cost Effectiveness analysis
• Cost Benefit Analysis
• Theoretical view of Cost Effectiveness Analysis
• CBA applicability for business
• Cost effectiveness and cost benefit studies
• Comparative analysis
• Horizontal analysis
• Ratio analysis
• Accounting principles
• Lecture Accounting principles
• Nguyên lý kế toán
• Correlation and path analysis
• Path analysis
• Path analysis study in Dolichos bean
• Genotypic correlations coefficient of yield
• Spatial pattern analysis
• Co localization analysis
• Interaction analysis
• Image analysis
• PALM single molecule microscopy
• Business analysis professional
• Strategy Analysis
• Requirements Analysis
• Requirements Life Cycle Management
• Business Analysis Planning
• BMC Genomics
• Ancestry inference
• Spatial analysis
• Inverse distance weighted interpolation
• Lecture Design and Analysis of Algorithms
• Bài giảng Thiết kế và phân tích thuật toán
• Analysis of Algorithms
• Design of Algorithms
• Running Time Analysis
• Analysis of 2 d Maxima
• Basic engineering circuit analysis
• Electric circuit analysis
• Electrical and computer engineering
• AC steady state analysis
• Steady state power analysis
• Magnetically coupled networks
• Software testing
• Software analysis
• Software testing and analysis
• Software process
• Software product
• Automating analysis