TAILIEUCHUNG - Báo cáo khoa học: Regulated expression by PPARa and unique localization of 17b-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver

17b-Hydroxysteroid dehydrogenase type 11 (17b-HSD11) is a member of the short-chain dehydrogenase⁄reductase family involved in the activation and inactivation of sex steroid hormones. We recently identified 17b-HSD11 as a gene that is efficiently regulated by peroxisome prolifera-tor-activated receptor-aPPARain the intestine and the liver [Motojima K (2004) Eur J Biochem271, 4141–4146]. | ỊFEBS Journal Regulated expression by PPARa and unique localization of 17p-hydroxysteroid dehydrogenase type 11 protein in mouse intestine and liver Yasuhide Yokoi Yuka Horiguchi Makoto Araki and Kiyoto Motojima Department of Biochemistry Meiji PharmaceuticalUniversity Kiyose Tokyo Japan Keywords hydroxysteroid dehydrogenase immunocytochemistry intestine lipid droplet proliferator-activated receptor Correspondence K. Motojima Department of Biochemistry Meiji PharmaceuticalUniversity 2-522-1 Noshio Kiyose Tokyo 204-8588 Japan Fax 81 42 495 8474 Tel 81 42 495 8474 E-mail motojima@ These authors contributed equally to this work Received 30 May 2007 revised 21 July 2007 accepted 24 July 2007 doi 17b-Hydroxysteroid dehydrogenase type 11 17P-HSD11 is a member of the short-chain dehydrogenase reductase family involved in the activation and inactivation of sex steroid hormones. We recently identified 17ft-HSD11 as a gene that is efficiently regulated by peroxisome prolifera-tor-activated receptor-a PPARa in the intestine and the liver Motojima K 2004 Eur J Biochem 271 4141-4146 . In this study we characterized 17P-HSD11 at the protein level to obtain information about its physiologic role in the intestine and liver. For this purpose specific antibodies against 17P-HSD11 were obtained. Western blotting analysis showed that administration of a peroxisome proliferator-activated receptor-a agonist induced 17P-HSD11 protein in the jejunum but not in the colon and to a much higher extent than in the liver of mice. A subcellular localization study using Chinese hamster ovary cells and green fluorescent protein-tagged 17P-HSD11 showed that it was mostly localized in the endoplasmic reticulum under normal conditions whereas it was concentrated on lipid droplets when they were induced. A pulse-chase experiment suggested that 17P-HSD11 was redistributed to the lipid droplets via the endoplasmic reticulum. Immunohistochemical analysis .

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