TAILIEUCHUNG - Báo cáo khoa học: Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes

Differential screening by PCR-select subtraction was carried out for cDNAs from leaves of red and green perilla, two chemovarietal forms of Perilla frutescensregarding anthocyanin accumulation. One hundred and twenty cDNA fragments were selected as the clones preferentially expressed in anthocyanin-accumulating red perilla over the nonaccumulating green perilla. | ễFEBS Journal Differential gene expression profiles of red and green forms of Perilla frutescens leading to comprehensive identification of anthocyanin biosynthetic genes Mami Yamazaki1 2 Masahisa Shibata1 Yasutaka Nishiyama1 3 Karin Springob1 f Masahiko Kitayama3 Norimoto Shimada4 Toshio Aoki4 Shin-ichi Ayabe4 and Kazuki Saito1 5 1 Graduate Schoolof PharmaceuticalSciences Chiba University Japan 2 CREST Japan Science and Technology Agency Kawaguchi Japan 3 Institute of Life Science Ehime Women s College Uwajima Japan 4 Department of Applied BiologicalSciences Nihon University Fujisawa Japan 5 RIKEN Plant Science Center Yokohama Japan Keywords anthocyanin chalcone isomerase glutathione S-transferase PCR-select subtraction Perilla frutescens Correspondence K. Saito Graduate Schoolof Pharmaceutical Sciences Chiba University Yayoi-cho 1-33 Inage-ku Chiba 263 8522 Japan Fax 81 43 290 2905 Tel 81 43 290 2904 E-mail ksaito@ Present address Ehime University Matsuyama Japan fDonald Danforth Plant Science Center St Louis MO USA Database The sequences reported in this article have been deposited in the DDBJ under the accession numbers AB362191 PfGSTD and AB362192 PfCHII Received 16 February 2008 revised 30 March 2008 accepted 7 May 2008 doi Differential screening by PCR-select subtraction was carried out for cDNAs from leaves of red and green perilla two chemovarietal forms of Perilla frutescens regarding anthocyanin accumulation. One hundred and twenty cDNA fragments were selected as the clones preferentially expressed in anthocyanin-accumulating red perilla over the nonaccumulating green perilla. About half of them were the cDNAs encoding the proteins related presumably to phenylpropanoid-derived metabolism. The cDNAs encoding glutathione S-transferase GST PfGSTl and chalcone isomerase CHI PfCHIl were further characterized. The expression of PfGSTl in an Ara-bidopsis thaliana tt19 mutant lacking the GST-like gene involved .

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