TAILIEUCHUNG - Báo cáo khoa học: Predicting the substrate specificity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit

The volatile compounds that constitute the fruit aroma of ripe tomato (Solanum lycopersicum) are often sequestered in glycosylated form. A homology-based screen was used to identify the geneSlUGT5, which is a member of UDP-glycosyltransferase 72 family and shows specificity towards a range of substrates, including flavonoid, flavanols, hydroqui-none, xenobiotics and chlorinated pollutants. | 1FEBS Journal Predicting the substrate specificity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit Thomas Louveau1 5 Celine Leitao1 6 Sol Green2 Cyril Hamiaux2 Benoit van der Rest1 Odile Dechy-Cabaret3 4 Ross G. Atkinson2 and Christian Chervin1 1 Université de Toulouse UMR Genomique et Biotechnologie des Fruits INRA-INP ENSAT Castanet-Tolosan France 2 The New Zealand Institute for Plant Food Research Ltd Auckland New Zealand 3 CNRS LCC Laboratoire de Chimie de Coordination Toulouse France 4 Universite de Toulouse UPS INP LCC Toulouse France 5 John Innes Centre Dep. Metabolic Biology Norwich UK 6 Universite de Strasbourg Equipe de Chimie Analytique des Molecules Bioactives Faculte de Pharmacie Illkirch France Keywords aroma docking eugenol guaiacol isosalicin methyl salicylate Correspondence C. Chenin ENSAT BP 32607 31326 Castanet-Tolosan France Fax 33 5 3432 3873 Tel 33 5 3432 3870 E-mail chervin@ Database Nucleotide sequence data have been submitted to the DDBJ EMBL GenBank databases under accession number HM209439 These authors contributed equally to this work Received 12 August 2010 revised 20 October 2010 accepted 12 November 2010 doi The volatile compounds that constitute the fruit aroma of ripe tomato Solanum lycopersicum are often sequestered in glycosylated form. A homology-based screen was used to identify the gene SlUGT5 which is a member of UDP-glycosyltransferase 72 family and shows specificity towards a range of substrates including flavonoid flavanols hydroquinone xenobiotics and chlorinated pollutants. SIUGT5 was shown to be expressed primarily in ripening fruit and flowers and mapped to chromosome I in a region containing a QTL that affected the content of guaiacol and eugenol in tomato crosses. Recombinant SlUGT5 protein demonstrated significant activity towards guaiacol and eugenol as well as benzyl alcohol and methyl salicylate however the highest in vitro .

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