TAILIEUCHUNG - Development of DNA barcodes for major insect pest of cole crops in MID-HILLS of Meghalaya

Significant morphological similarities within or in between species of insects has made reliable taxonomic identification difficult. DNA barcoding has appeared to be a useful tool in resolving the issues related to the identification of taxonomically difficult insect species. The Northeastern region of India is one of the mega biodiversity hotspots in the World and the climatic conditions of the region are highly conducive for reproduction and multiplication of insects. The efforts were undertaken to study the biodiversity of insects and to develop molecular database by developing DNA barcodes of major insect pests of cole crops in mid hills of Meghalaya. Limited information is available on insect pests in cole crops ecosystem in mid –hills of Meghalaya. A total of 12 insect pests belonging to four insect orders viz., Lepidoptera (6), Coleoptera (2), Diptera (2), Hemiptera (2) were documented during the year 2014-2015. The insect species viz., P. brassicae, Spodopteralitura, Myzus persicae and Plutella xylostella were found to be a major pest in this region. The collected species were identified by established taxonomic keys, by taxonomists and/or molecular basis. DNA was successfully extracted from multiple specimens of 12 insect species and molecular assays were also undertaken for presence of Wolbachia infection. The Wolbachia infected specimens were discarded and not used for further analysis. | Development of DNA barcodes for major insect pest of cole crops in MID-HILLS of Meghalaya

• Sinh học
• DNA barcoding
• Lepidopteran insects and mtDNA
• Cole crops in MID HILLS
• MID HILLS of Meghalaya
• DNA barcodes
• Major insect pest
• Potential DNA barcoding loci
• Plastid genome
• Intraspecies discrimination in rice
• DNA barcoding in rice
• Gulf of mannar
• DNA barcoding on cardinalfishes
• Thoothukudi coast
• DNA barcoding for identification
• Identification of some fish species (Carangidae)
• Vietnam coastal area
• Morphological classification method
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Indian wild coffee species
• Unique fixed nucleotides
• DNA sequence polymorphism
• BMC Plant Biology
• Low copy nuclear gene
• Plant collections
• Ecological diversity
• BMC Genomics
• DNA barcode
• High throughput sequencing
• COI barcode region
• Fungi symbionts
• Tạp chí khoa học
• Công nghệ sinh học
• Khoa học tự nhiên
• Dendrobium transparens
• Pitcher plant
• Cytochrome oxidase I
• Nepenthes genus
• Molecular identification
• Bayesian inference
• Maximum likelihood
• Medicinal plants
• Journal of Zoology
• Cytochrome oxidase I COI
• Black cherry aphid
• Nucleotide diversity
• Plant Biology
• Super barcoding
• Calypogeia species
• DNA fragments
• Multi locus barcoding
• Adinandra lienii
• Mã vạch DNA matK
• Trình tự đoạn gen matK phân lập
• Chi Adinandra thuộc họ Chè Theaceae
• Hỗn hợp phản ứng PCR
• BMC Genetics
• BOLD systems
• Fungal taxonomy
• DNA barcoding technique
• Trình tự gen RpoC1
• Gen RpoC1
• Gen lục lạp
• Xác định quan hệ chủng loài
• Kỹ thuật DNA mã vạch
• Genome Biology
• Assembly free
• Alignment free
• Genome skimming
• DNA reference database
• Second generation sequencing
• P corylifoilia
• M pruriens
• C ternatea
• TrnH psbA
• Species Identification
• Trình tự gen rpoB
• Morinda officinalism
• Ngân hàng gen mã số KR689730
• PCR genotyping
• Identification of lichens
• Advancements in methods
• Various natural compounds
• BMC Bioinformatics
• Cellular barcoding
• Lineage tracing
• Next generation sequencing
• Sequencing error
• PCR error
• COI gene
• Intra and inter species diversity
• Tropical forest
• Genetic distance of leaf beetles
• Journal of Botany
• Hypericum occurring in Sicily
• Hypericum triquetrifolium
• Phytotherapic purposes
• Molecular analyses
• Tyrrhenian Sea
• Cryptic diversity
• Rare species
• Cytochrome c oxidase subunit I (COI)
• Luận văn Thạc sĩ
• Luận văn Thạc sĩ Sinh học
• Rong biển thuộc chi Ulva
• Sinh học thực nghiệm
• Sinh học phân tử DNA barcoding
• Phương pháp điện di Gel agarose
• Approximate Bayesian computation
• Freshwater species
• Neocaridina species