TAILIEUCHUNG - DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam

Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives. DNA barcodes promise to be a precise and reliable tool for distinguishing the processed Huyen-sam materials from their counterfeits. However, studies about using DNA barcodes for classification of Scrophularia L. in Vietnam are not available. Here, we conducted a taxonomic analysis of eight Scrophularia L. samples collected from the mountain areas of Northern Vietnam. Based on the combined sequence data of ribosomal nuclear ITS, a part of chloroplast rbcL gene and trnL-trnF intergenic spacer, phylograms of Scrophularia L. were generated by both Bayesian inference and maximum likelihood bootstrap method. The phylogenetic analysis showed that the tested samples have a sister relationship to S. ningpoensis. Hopefully, the analysis strategy that we used would contribute to further phylogenetic analyses of medicinal plants of Vietnam in the future. | DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam

• Sinh học
• Molecular identification
• DNA barcoding
• Bayesian inference
• Maximum likelihood
• Medicinal plants
• Rhinoestrus spp larvae
• Molecular and morphological identification
• Stage larvae
• Rhinoestrus spp
• Arbuscular mycorrhizal
• Morphological and molecular identification
• Compound microscope
• Important fungi for agriculture
• Compound feed
• Aspergillus species
• Different sources of animals feed
• Molecular identification of Aspergillus spp
• DNA barcode Orchids
• DNA fingerprinting
• Molecular identification orchidaceae
• Molecular markers
• Molecular phylogeny
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• Their seasonal abundance
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• Based on barcoding
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• Molecular technique
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• Molecular characteristics
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• Identification of panax species in Vietnam
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