TAILIEUCHUNG - Functional analysis of recombinant codon-optimized bovine neutrophil b-defensin

Defensins are cationic antimicrobial peptides with a broad range of activities against bacteria and fungi. In the present study, the entire coding sequence of codon-optimized Bovine Neutrophil b-Defensin 2 (BNBD2) was designed and placed upstream of Trx coding sequence into the pET-48b (+) vector. Furthermore, the codon-optimized pelB signal sequences were also added to the upstream of BNBD2 for periplasmic localization. The periplasmic sorting of recombinant b-Defensin 2 was evaluated by osmotic shock and SDS–PAGE on the released proteins. Moreover, the expression of BNBD2-Trx fusion protein was confirmed by the Western blotting technique. Next, the purification of recombinant protein was achieved by Ni++ affinity chromatography. BNBD2 was also separated from Trx by chemical cleavage with formic acid. Finally, both of the antibacterial and antifungal activities of the purified protein were examined. Overall, the results indicated successful periplasmic production of BNBD2 protein, which showed antifungal activity against some of Aspergillus species as well as the antibacterial activity, expressed as successfully suppressed growth of Escherichia coli and Staphylococcus aureus. | Functional analysis of recombinant codon-optimized bovine neutrophil b-defensin

• Sinh học
• Antimicrobial peptides
• Bovine neutrophil beta Defensin 2
• Codon optimization
• Periplasmic expression
• Recombinant codon optimized
• Fish disease
• Therapeutic alternative
• Hyalophora cecropia
• BMC Genetics
• Drosophila melanogaster
• Amyloid β peptides
• Transcription factor FOXO
• Peroxiredoxin 5
• Biochemistry
• molecular Biology
• biological cells
• innate immunity
• cytoplasm
• BMC Genomics
• Feature weighting
• Molecular descriptors
• Multi objective evolutionary algorithm
• Peptide representation
• Antimicrobial resistance
• Antimicrobial peptide
• Deep learning
• Feature fusion
• Organisms against microbes
• scientific reports
• thesis report
• medical report
• medical knowledge
• medical research
• analysis of cytoplasmic
• breast cancer
• medicine
• chemical reaction
• Crystal structure
• medical analysis
• biochemical studies
• BMC Plant Biology
• Plant immunity
• Physcomitrella patens
• Cryptic peptides
• Functionally active proteins
• Intercellular fluid
• Cysteine rich peptides
• Heterologous expression
• Transgenic plants
• Vacuum infiltration
• Data independent acquisition
• Trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Report medicine
• traditional medicine
• Cell penetrating peptides
• Antimicrobial peptide LL 37
• RNA interference
• Scanning tunneling electron microscopy
• Protein bodies
• Pathogen resistance
• Fusarium verticillioides
• Dickeya dadantii
• Oryza sativa
• BMC Musculoskeletal Disorders
• Multiple trauma
• Human beta defensin
• Real time PCR
• Parallel identification
• Signal peptide region
• Journal of Zoology
• Foam nest
• Mass spectrometry
• Protein profiling
• Biosynthesis gene clusters
• Genome mining
• Ribosomally synthesized post translationally modified peptides
• Non ribosomal peptides
• immune system
• Antimicrobial
• biosynthesis
• scientific research
• Equus asinus
• genetics
• Structural biochemistry
• Structural Biology
• tumor cells