TAILIEUCHUNG - Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes

Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Leuven, Belgium; 2Institut de Pharmacologie et ´ Biologie Structurale, Unite Mixte de Recherche Centre National de la Recherche Scientifique 5089, Toulouse, France; 3Institute of Plant Biochemistry, Halle, Germany; 4Laboratory of Developmental Physiology and Molecular Biology, Katholieke Universiteit Leuven, Leuven, Belgium | Eur. J. Biochem. 268 6263-6273 2001 FEBS 2001 Purification characterization immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium hedge bindweed rhizomes Els J. M. Van Damme1 Jialiang Hu1 Annick Barre2 Bettina Hause3 Geert Baggerman4 Pierre Rouge2 and Willy J. Peumans1 1 Laboratory for Phytopathology and Plant Protection Katholieke Universiteit Leuven Leuven Belgium 2Institut de Pharmacologie et Biologie Structurale Unite Mixte de Recherche Centre National de la Recherche Scienti que 5089 Toulouse France 3Institute of Plant Biochemistry Halle Germany 4 Laboratory of Developmental Physiology and Molecular Biology Katholieke Universiteit Leuven Leuven Belgium An abundant catalytically active p-amylase EC was isolated from resting rhizomes of hedge bindweed Calystegia sepium . Biochemical analysis of the purified protein molecular modeling and cloning of the corresponding gene indicated that this enzyme resembles previously characterized plant p-amylases with regard to its amino-acid sequence molecular structure and catalytic activities. Immunolocalization demonstrated that the p-amylase is exclusively located in the cytoplasm. It is suggested that the hedge bindweed rhizome p-amylase is a cytoplasmic vegetative storage protein. Keywords p-amylase Calystegia sepium hedge bindweed immunolocalization vegetative storage protein. Exo-hydrolases catalyzing the release of p-maltose from the nonreducing ends of a-1 4-linked oligo- and polyglucans also so-called p- or exo-amylases EC have been studied for several decades because they are possibly involved in starch metabolism in plants and play an important role in biotechnological processes whereby starch is converted into simple sugars. In the past research on p-amylases has been focussed on the abundant p-amylases found in the endosperm of barley Hordeum vulgare and some other cereals 1 soybean Glycine max seeds 2 and sweet potato Ipomoea batatas tubers 3 .

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• Report medicine
• traditional medicine
• scientific reports
• Crystal structure
• medical analysis
• biochemical studies
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• environmental medicine