TAILIEUCHUNG - Báo cáo Y học: A Ca2+/CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter

An immuno-homologue of maize Ca2+/calmodulin (CaM)dependent protein kinase with a molecular mass of 72 kDa was identified in pea. The pea kinase (PsCCaMK) was upregulated in roots in response to low temperature and increased salinity. Exogenous Ca2+ application increased the kinase level and the response was faster than that obtained following stress application. Low temperaturemediated, but not salinity-mediated stress kinase increase was inhibited by the application of EGTA and W7, a CaM inhibitor | Eur. J. Biochem. 269 3193-3204 2002 FEBS 2002 doi A Ca2 CaM-dependent kinase from pea is stress regulated and in vitro phosphorylates a protein that binds to AtCaM5 promoter Sona Pandey Shiv B. Tiwarjt Wricha Tyagi Mali K. Reddy Kailash C. Upadhyaya and Sudhir K. Sopory School of Life Sciences Jawaharlal Nehru University New Delhi India and International Center for Genetic Engineering and Biotechnology New Delhi India An immuno-homologue of maize Ca2 calmodulin CaM -dependent protein kinase with a molecular mass of 72 kDa was identified in pea. The pea kinase PsCCaMK was upregulated in roots in response to low temperature and increased salinity. Exogenous Ca2 application increased the kinase level and the response was faster than that obtained following stress application. Low temperature-mediated but not salinity-mediated stress kinase increase was inhibited by the application of EGTA and W7 a CaM inhibitor. The purification of PsCCaMK using immunoaffinity chromatography resulted in coelution of the kinase with another polypeptide of molecular mass 40 kDa p40 . Western blot revealed the presence of PsCCaMK in nuclear protein extracts and was found to phosphorylate p40 in vitro. Gel mobility shift and South-Western analysis showed that p40 is a DNA-binding protein and it interacted specifically with one of the cis acting elements of the Arabidopsis CaM5 gene AtCaM5 promoter. The binding of p40 to the specific elements in the AtCaM5 promoter was dependent of its dephosphorylated state. Our results suggest that p40 could be an upstream signal component of the stress responses. Keywords calmodulin DNA-protein interaction plant protein kinase protein phosphorylation stress signaling. Plants perceive a variety of signals from the external environment as well as from the internal cellular milieu generated during various developmental processes. Signals such as light nutrients and various environmental stresses etc. are perceived by .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• Structural biochemistry
• bacteria
• chemical reaction
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine