TAILIEUCHUNG - Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo

Mitochondrial uncoupling protein 1 (UCP1) is a speci®c marker of multilocular brown adipocytes. Ectopic UCP1 in white fat of aP2-Ucp1micemitigates development of obesity byboth, increasing energy expenditure anddecreasingin situ lipogenesis. In order to further analyse consequences of respiratory uncoupling inwhite fat, the e ects of the ectopic UCP1 on the morphology of adipocytes and biogenesis of mitochondria in these cells were studied. | Eur. J. Biochem. 269 19-28 2002 FEBS 2002 Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo Martin Rossmeisl1 Gioraio Barbatelli2 Pavel Flachs1 Petr Brauner1 Maria Cristina Zinaaretti2 M B B FF FF B F Ff B ww B Ff Ff fB B W fB BF B B B F B FF w FF B B B FF B B F B FF B Ff B FF w B B FF B F B B B FF B B FF BFB B B B B FF B B B B fF ff B FF B F Mariella Marelli2 Petra Janovska1 Milada Horáková1 Ivo Svrovv1. Saverio Cinti2 and Jan Kopeck 1 F F F F 1Department of Adipose Tissue Biology and Center for Integrated Genomics Institute of Physiology Academy of Sciences of the Czech Republic Prague Czech Republic 2Institute of Anatomy University of Ancona Italy Mitochondrial uncoupling protein 1 UCP1 is a specific marker of multilocular brown adipocytes. Ectopic UCP1 in white fat of aP2- Ucp1 mice mitigates development of obesity by both increasing energy expenditure and decreasing in situ lipogenesis. In order to further analyse consequences of respiratory uncoupling in white fat the effects of the ectopic UCP1 on the morphology of adipocytes and biogenesis of mitochondria in these cells were studied. In subcutaneous white fat of both aP2- Ucp1 and young control 5-week-old mice numerous multilocular adipocytes were found while they were absent in adult 7- to 9-month-old animals. Only unilocular cells were present in epididymal fat of both genotypes. In both fat depots of aP2- Ucp1 mice the levels of the UCP1 transcript and UCP1 antigen declined during ageing and they were higher in subcutaneous than in epididymal fat. Under no circumstances could ectopic UCP1 induce the conversion of unilocular into multilocular adipocytes. Presence of ectopic UCP1 in unilocular adipocytes was associated with the elevation of the transcripts for UCP2 and for subunit IV of mitochondrial cytochrome oxidase COX IV and increased content of mitochondrial cytochromes. Electron microscopy indicated changes of .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• intermediate cells
• cell proliferation
• medical analysis
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine