TAILIEUCHUNG - Production of transgenic deepwater indica rice plants expressing a synthetic Bacillus thuringiensis cryIA(b) gene with enhanced resistance to yellow stem borer

Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation. The presence and expression of the Bt gene in regenerated plants were confirmed by Southern and Western blot analyses. Inheritance of the transgene was confirmed in the T1 generation. Insect bioassays showed. | ELSEVIER Plant Science 135 1998 25-30 Production of transgenic deepwater indica rice plants expressing a synthetic Bacillus thuringiensis cryIA b gene with enhanced resistance to yellow stem borer Mohammad Firoz Alam Karabi Datta Editha Abrigo Alelie Vasquez Dharmawansa Senadhira Swapan K. Datta Plant Breeding Genetics and Biochemistry Division International Rice Research Institute . Box 933 Manila 1099 Philippines Received 14 October 1997 received in revised form 17 December 1997 accepted 23 February 1998 Abstract Yellow stem borer has been identified as a major insect pest of deepwater rice causing severe yield losses. Bt gene s from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene s is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA b gene into embryogenic calli of a deepwater indica rice variety Vaidehi by using the biolistic method of transformation. The presence and expression of the Bt gene in regenerated plants were confirmed by Southern and Western blot analyses. Inheritance of the transgene was confirmed in the T1 generation. Insect bioassays showed an enhancement of resistance against the yellow stem borer. This is the first report of lowland rice engineered with Bt gene. 1998 Elsevier Science Ireland Ltd. All rights reserved. Keywords Transgenic deepwater rice cryIA b gene Yellow stem borer resistance 1. Introduction Deepwater rice DWR is grown on about 9 million hectares of flooded lands in Asia the river Abbreviations 2 4-D 2 4-Dichlorophenoxy acetic acid NAA 1-a Napthalene acetic acid. Corresponding author. Tel. 63 2 8450563 8450569 fax 63 2 7612406 8450606 e-mail sdatta@ basins of Ganges-Brahmaputra of India and Bangladesh the Irrawaddy of Myanmar the Mekong of Vietnam and Cambodia and the Chao Phraya of Thailand and West Africa upper and middle basins of the Niger River 1 . Farmers in .

• Báo cáo khoa học
• Transgenic deepwater rice; cryIA(b) gene; Yellow stem borer resistance
• Genotype and plasmids
• Insect bioassay
• Genomic DNA
• Simple genomic DNA extraction
• Efficient and universal method
• The extraction of genomic DNA
• Genomic DNA from bacteria
• Microalgae suitable
• PCR based applications
• DNA tái tổ hợp
• công nghệ gen
• kỹ thuật di truyền
• kỹ thuật gen
• khoa học tự nhiên
• sinh học
• Pigeon pea
• Genomic DNA extraction
• Genomic DNA extraction from pigeon pea
• Rapid procedure
• Showed highly pure DNA
• Oryza sativa
• Solanum lycopersicum
• DNA extraction
• DNA purity
• Thư viện DNA bộ gene
• Bài giảng Thư viện DNA bộ gen
• Thư viện DNA
• Sàng lọc dòng DNA
• DNA genomic library
• Thử miễn dịch
• Genomic DNA isolation
• Guanidinium isothiocyanate
• DNA isolation methods
• Microbiology laboratories
• Staphylococcus aureus
• Rapid and efficient procedure
• Trichoderma spp
• DNA with high yield
• PCR purposes
• BMC Bioinformatics
• Comparative genomics
• Alignment free
• Genomic signature
• Information distance
• Additive DNA signature
• Composite DNA signature
• Assembled DNA signature
• BMC Genetics
• DNA methylation
• 5 azacytidine
• Genomic binding
• Remodel chromatin
• DNA demethylating reagent
• BMC Genomics
• DNA epigenome
• DNA N6 methyladenosine
• DNA N4 methylcytosine
• Biological functions
• báo cáo thực tập
• luận văn tốt nghiệp
• thư viện cDNA
• thư viện genomic DNA
• Lai insitu
• hoạt tính enzym
• Journal of Botany
• Aluminum stress
• iPBS profile
• Genomic instability
• Reactive oxygen species
• Primary osteoporosis
• Bone remodeling
• Osteoporotic fracture bone tissues
• DNA sequencing
• Molecular cytogenetic identification
• Wheat–Thinopyrum intermedium introgression line
• Total genomic DNA
• Wheatgrass chromosomes
• ABD genomic DNA
• SEQUENCING REACTION
• STRAND SEPARATION
• PRIMER ANNEALING
• PRIMER EXTENSION
• DNA PREPARATION
• Phenotypic plasticity
• Genomic data
• Sociality through DNA Methylation
• Epigenetic control of sociality
• Ancient DNA
• DNA contamination removal
• Competitive mapping
• Cost effective sequencing
• High throughput sequencing
• Genome Biology
• Genomic imprinting
• Oocyte specific epigenetic
• Epigenetic phenomenon
• Bimodal methylation
• Cinnamomum verum Presl
• Cinnamomum sp
• Cinnamomum species
• Commercial DNA isolation kits
• Yield DNA
• Species concept
• Genome sequences
• DNA DNA hybridization
• Prokaryotic species
• Preimplantation embryos
• Epigenetic reprogramming
• DNA methyltransferases
• 6mA modification
• DNA variation
• RNA variation
• Transmitted variation
• Genomic feature analysis
• Information theory
• Mutual Information
• Gene ranking
• Methylation analysis
• Clinico genomic model
• High dimensional data
• Logistic regression