TAILIEUCHUNG - Báo cáo khoa học: THE THEODOR BU¨CHER LECTURE Investigating signal transduction with genetically encoded fluorescent probes

Ca 2+ and cAMP are ubiquitous second messengers in euk-aryotes and control numerous physiological responses ran-ging fromfertilization to cell death induction 1 . Todistinguish between these different responses, their subtle regulation in time, space and amplitude is needed. Therefore, the char-acterization of the signalling process requires measurement of secondmessengers with tools of precise localization, high dynamic range and as little disturbance of cell physiology as possible. | Eur. J. Biochem. 270 2343-2352 2003 FEBS 2003 doi THE THEODOR BUCHER LECTURE Investigating signal transduction with genetically encoded fluorescent probes Delivered on 22 October 2002 at the 28th FEBS Meeting in Istanbul Tullio Pozzan1 2 Marco Mongillo2 and Riidiger Rudolf1 1 Department of Biomedical Sciences CNR Institute of Neurosciences University of Padua Italy 2 Venetian Institute for Molecular Medicine Padua Italy Ca2 and cAMP are ubiquitous second messengers in eukaryotes and control numerous physiological responses ranging from fertilization to cell death induction. To distinguish between these different responses their subtle regulation in time space and amplitude is needed. Therefore the characterization of the signalling process requires measurement of second messengers with tools of precise localization high dynamic range and as little disturbance of cell physiology as possible. Recently fluorescent proteins of marine jellyfish have given rise to a set of genetically encoded biosensors which fulfil these criteria and which have already led to important new insights into the subcellular handling of Ca2 and cAMP. The use of these probes in combination with new microscopical methods such as two-photon microscopy now enables researchers to study second messenger signalling in intact tissues. In this review the genetically encoded measurement probes and their origin are briefly introduced and some recent insights into the spatio-temporal complexity of both Ca2 and cAMP signalling obtained with these tools are discussed. Second messengers and the encoding paradigm Cells are structures of immense spatio-temporal complexity. Myriads of different proteins fatty acids carbohydrates and other organic and inorganic compounds are being produced degraded or transported at each moment. To enhance their reliability and efficiency these processes are organized in a highly complex and dynamic system of subcellular compartments. These .

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