TAILIEUCHUNG - Báo cáo khoa học: The role of Ureaplasma nucleoside monophosphate kinases in the synthesis of nucleoside triphosphates

Mollicutes are wall-less bacteria and cause various diseases in humans, ani-mals and plants. They have the smallest genomes with low G + C content and lack many genes of DNA, RNA and protein precursor biosynthesis. Nucleoside diphosphate kinase (NDK), a house-keeping enzyme that plays a critical role in the synthesis of nucleic acids precursors, . | ỊFEBS Journal The role of Ureaplasma nucleoside monophosphate kinases in the synthesis of nucleoside triphosphates Liya Wang Department of Molecular Biosciences Swedish University of AgriculturalSciences The BiomedicalCentre Uppsala Sweden Keywords Mollicutes nucleotide biosynthesis nucleoside diphosphate kinase nucleoside monophosphate kinase Ureaplasma Correspondence L. Wang Department of Molecular Biosciences Section of Veterinary Medical Biochemistry Swedish University of AgriculturalSciences The Biomedical Centre PO Box 575 SE-751 23 Uppsala Sweden Fax 46 18550762 Tel 46 184714119 E-mail Received 11 January 2007 revised 5 February 2007 accepted 14 February 2007 doi Mollicutes are wall-less bacteria and cause various diseases in humans animals and plants. They have the smallest genomes with low G C content and lack many genes of DNA RNA and protein precursor biosynthesis. Nucleoside diphosphate kinase NDK a house-keeping enzyme that plays a critical role in the synthesis of nucleic acids precursors . NTPs and dNTPs is absent in all the Mollicutes genomes sequenced to date. Therefore it would be of interest to know how Mollicutes synthesize dNTPs NTPs without NDK. To answer this question nucleoside monophosphate kinases NMPKs from Ureaplasma were studied regarding their role in the synthesis of NTPs dNTPs. In this work Ureaplasma adenylate kinase cytidylate kinase uridylate kinase and thymidylate kinase were cloned and expressed in Escherichia coli. The recombinant enzymes were purified and characterized. These NMPKs are base specific as indicated by their names and capable of converting d NMPs directly to d NTPs. The catalytic rates of d NTPs and d NDP synthesis by these NMPKs were determined using tritium-labelled d NMPs and the rates for d NDP synthesis in general were much higher up to 100-fold than that of d NTP. Equilibrium studies with adenylate kinase suggested that the rates of NTPs dNTPs .

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