TAILIEUCHUNG - Báo cáo khoa học: Alcohol linked to enhanced angiogenesis in rat model of choroidal neovascularization

One of the pathologic complications of exudative (. wet-type) age-related macular degeneration (AMD) is choroidal neovascularization (CNV). The aim of this study was to investigate whether chronic and heavy alcohol consumption influenced the development of CNV in a rat model. | ềFEBS Journal Alcohol linked to enhanced angiogenesis in rat model of choroidal neovascularization Puran S. Bora Sankaranarayanan Kaliappan Qin Xu Shalesh Kumar Yali Wang Henry J. Kaplan and Nalini S. Bora Department of Ophthalmology and VisualScience Kentucky Lions Eye Center University of Louisville KY USA Keywords adiponectin angiogenesis choroids macular degeneration neovascularization Correspondence P. S. Bora Department of Ophthalmology Jones Eye Institute University of Arkansas for MedicalSciences 4301 West Markham 523 Little Rock AR 72205 USA Fax 1 501 686 7037 Tel 1 501 686 5150 E-mail pbora@ Present address Jones Eye Institute University of Arkansas for MedicalSciences Little Rock AR USA Received 17 January 2006 accepted 2 February 2006 doi One of the pathologic complications of exudative . wet-type age-related macular degeneration AMD is choroidal neovascularization CNV . The aim of this study was to investigate whether chronic and heavy alcohol consumption influenced the development of CNV in a rat model. The oxidative metabolism of alcohol is minimal or absent in the eye so that ethanol is metabolized via a nonoxidative pathway to form fatty acid ethyl esters FAEE . Fatty acid ethyl ester synthase FAEES was purified from the choroid of Brown Norway BN rats. The purified protein was 60 kDa in size and the antibody raised against this protein showed a single band on western blot. BN rats on a regular diet were fed alcohol for 10 weeks. Control rats were fed water with a regular diet and pair-fed control rats were fed regular diet water and glucose. We found that FAEES activity was increased in the choroid of alcohol-treated rats compared with controls. The amount of ethyl esters produced in the choroid of 10 week alcohol-fed rats was more than rats fed alcohol for 1 week. The increased accumulation of ethyl esters was associated with a increased expression of cyclin E and cyclin E .

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