TAILIEUCHUNG - Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays

Complex I (NADH: ubiquinone oxidoreductase) is the first complex in the respiratory electron transport chain. Homologs of this complex exist in bacteria, mitochondria and chloroplasts. The minimal complex I from mitochondria and bacteria contains 14 different subunits grouped into three modules: membrane, connecting, and soluble subcomplexes. The com-plex I homolog (NADH dehydrogenase or Ndh complex) from chloroplasts from higher plants contains genes for two out of three modules: the mem-brane and connecting subcomplexes. . | ềFEBS Journal Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays Costel C. Darie1 Martin L. Biniossek2 Veronika Winter3 Bettina Mutschler4 and Wolfgang Haehnel4 1 Brookdale Department Molecular Celland DevelopmentalBiology Mount Sinai Schoolof Medicine New York USA 2 Institut fuer Molekulare Medizin und Zellforschung Albert-Ludwigs Universitaet Freiburg Germany 3 Departement de Biologie Moleculaire Universite de Geneve Switzerland 4 Institut fuer Biologie II Biochemie der Pflanzen Albert-Ludwigs Universitaet Freiburg Germany Keywords chloroplast maize mass spectrometry native electrophoresis Ndh complex Correspondence C. C. Darie Brookdale Department of Molecular Celland DevelopmentalBiology Annenberg Building Box1020 Mount Sinai Schoolof Medicine One Gustave L. Levy Place New York NY 10029-6574 USA Fax 1 718 246 2616 Tel 1 212 241 8620 E-mail Received 31 January 2005 revised 23 March 2005 accepted 24 March 2005 doi Complex I NADH ubiquinone oxidoreductase is the first complex in the respiratory electron transport chain. Homologs of this complex exist in bacteria mitochondria and chloroplasts. The minimal complex I from mitochondria and bacteria contains 14 different subunits grouped into three modules membrane connecting and soluble subcomplexes. The complex I homolog NADH dehydrogenase or Ndh complex from chloroplasts from higher plants contains genes for two out of three modules the membrane and connecting subcomplexes. However there is not much information about the existence of the soluble subcomplex which is the electron input device in bacterial complex I in the composition of the Ndh complex. Furthermore there are contrasting reports regarding the subunit composition of the Ndh complex and its molecular mass. By using blue native BN PAGE and Tricine PAGE or colorless-native CN PAGE BN PAGE and Tricine PAGE. combined with mass .

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