TAILIEUCHUNG - Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2

3-carboxy-cis,cis-muconate lactonizing enzymes participate in the protoca-techuate branch of the 3-oxoadipate pathway of various aerobic bacteria. The gene encoding a 3-carboxy-cis,cis-muconate lactonizing enzyme (pcaB1S2) was cloned from a gene cluster involved in protocatechuate deg-radation by Agrobacterium radiobacterstrain S2. | ỊFEBS Journal Structure and function of the 3-carboxy-cis cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 Carl I I I l 1 I íírí I hti rS2 3 Ta ms r Doq o1T Rihx lli R mar1 K affhiaQ i nntTPn1 Anri . R 0I71 oau ndi f Lail Lciiuu f Idiuaia Daota f Uiuyiic Duiyci f Iviatuiiao VUIILZCII f M luicdo otuiz and Adrian Goldman2 1 Institut fur Mikrobiologie Universitat Stuttgart Germany 2 Institute of Biotechnology University of Helsinki Finland 3 NationalGraduate School in Informationaland StructuralBiology Abo Akademi University Finland Keywords Agrobacterium b-ketoadipate pathway 3-carboxy-cis cis-muconate lactonizing enzyme fumarase II family Correspondence A. Goldman Institute of Biotechnology University of Helsinki PO Box 65 00014 HY Finland Fax 358 9 191 59940 Tel 358 9 191 58923 E-mail A. Stolz Institut fur Mikrobiologie Universitat Stuttgart Allmandring 31 70569 Stuttgart Germany Fax 49 711 685 6 5725 Tel 49 711 685 6 5489 E-mail These authors contributed equally to this work Present address Institut Pasteur Paris France Chemisches und Veterinaruntersuchungsamt Stuttgart Fellbach Germany Received 8 August 2006 revised 22 September 2006 accepted 25 September 2006 doi 3-carboxy-cis cis-muconate lactonizing enzymes participate in the protoca-techuate branch of the 3-oxoadipate pathway of various aerobic bacteria. The gene encoding a 3-carboxy-cis cis-muconate lactonizing enzyme pcaB1S2 was cloned from a gene cluster involved in protocatechuate degradation by Agrobacterium radiobacter strain S2. This gene encoded for a 3-carboxy-cis cis-muconate lactonizing enzyme of 353 amino acids - significantly smaller than all previously studied 3-carboxy-cis cis-muconate lact-onizing enzymes. This enzyme ArCMLE1 was produced in Escherichia coli and shown to convert not only 3-carboxy-cis cis-muconate but also .

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