TAILIEUCHUNG - Báo cáo khoa học: Cloning of type 1 cannabinoid receptor in Rana esculenta reveals differences between genomic sequence and cDNA

The endocannabinoid system is a conserved system involved in the modula-tion of several physiologic processes, from the activity of the central ner-vous system to reproduction. Type 1 cannabinoid receptor (CNR1) cDNA was cloned from the brain and testis of the anuran amphibian, the frog Rana esculenta. | ỊFEBS Journal Cloning of type 1 cannabinoid receptor in Rana esculenta reveals differences between genomic sequence and cDNA Rosaria Meccariello1 Rosanna Chianese2 Gilda Cobellis2 Riccardo Pierantoni2 and Silvia Fasano2 1 Dipartimento di Studi delle Istituzioni e dei Sistemi Territorial University di Napoli Parthenope Naples Italy 2 Dipartimento di Medicina Sperimentale Sez. F. Bottazzi II University di Napoli Naples Italy Keywords cannabinoid receptor frog nonsynonymous mutations post-transcriptionalmodifications synonymous mutations Correspondence R. Pierantoni Dipartimento di Medicina Sperimentale Sez. F. Bottazzi II Universita di Napoli via Costantinopoli 16 80138 Napoli Italy Fax 39 081 5667536 00 Tel 39 081 5667617 E-mail Database The sequences reported in this paper have been deposited in the GenBank database under the accession numbers AM113546 AM260468 and AM260467 for frog cnr1 brain testis cDNA and genomic DNA respectively Received 8 January 2007 revised 3 April 2007 accepted 5 April 2007 doi The endocannabinoid system is a conserved system involved in the modulation of several physiologic processes from the activity of the central nervous system to reproduction. Type 1 cannabinoid receptor CNR1 cDNA was cloned from the brain and testis of the anuran amphibian the frog Rana esculenta. Nucleotide identity ranging from to is observed among vertebrates. The reading frame encoded a protein of 462 amino acids FCNR1 with all the properties of a membrane G-coupled receptor. Alignments of FCNR1 with those of other vertebrates revealed amino acid identity ranging from to critical domains for CNR1 functionality were conserved in the frog. As nucleotide differences of cnrl cDNA were observed in brain and testis the genomic sequence of the cnrl gene was also determined in the same tissue preparations. Nucleotide changes in codons 5 30 70 186 252 and 408 were observed when cDNA and .

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