TAILIEUCHUNG - Báo cáo khoa học: N-Glycans of the porcine nematode parasite Ascaris suum are modified with phosphorylcholine and core fucose residues

In recent years, the glycoconjugates of many parasitic nematodes have attracted interest due to their immunogenic and immunomodulatory nat-ure. Previous studies with the porcine roundworm parasiteAscaris suum have focused on its glycosphingolipids, which were found, in part, to be modified by phosphorylcholine. | ễFEBS Journal N-Glycans of the porcine nematode parasite Ascaris suum are modified with phosphorylcholine and core fucose residues Gerald Poltl Denise Kerner Katharina Paschinger and Iain B. H. Wilson Department fur Chemie Universitat fur Bodenkultur Vienna Austria Keywords Ascaris fucose nematode N-glycan parasite phosphorylcholine Correspondence I. B. H. Wilson Department fur Chemie Universitat fur Bodenkultur A-1190 Wien Austria Fax 43 1 360066059 Tel 43 1 360066541 E-mail Received 14 August 2006 revised 21 November 2006 accepted 23 November 2006 doi In recent years the glycoconjugates of many parasitic nematodes have attracted interest due to their immunogenic and immunomodulatory nature. Previous studies with the porcine roundworm parasite Ascaris suum have focused on its glycosphingolipids which were found in part to be modified by phosphorylcholine. Using mass spectrometry and western blotting we have now analyzed the peptide N-glycosidase A-released N-glycans of adults of this species. The presence of hybrid bi- and triantennary N-gly-cans some modified by core a1 6-fucose and peripheral phosphorylcholine was demonstrated by LC electrospray ionization ESI -Q-TOF-MS MS as was the presence of paucimannosidic N-glycans some of which carry core a1 3-fucose and oligomannosidic oligosaccharides. Western blotting verified the presence of protein-bound phosphorylcholine and core a1 3-fucose whereas glycosyltransferase assays showed the presence of core a1 6-fuco-syltransferase and Lewis-type a1 3-fucosyltransferase activities. Although the unusual tri- and tetrafucosylated glycans found in the model nematode Caenorhabditis elegans were not found the vast majority of the N-glycans found in A. suum represent a subset of those found in C. elegans thus our data demonstrate that the latter is an interesting glycobiological model for parasitic nematodes. Ascaris suum is one of a number of nematode parasites which .

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