TAILIEUCHUNG - Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery

Hydrogenophilus thermoluteoluscytochromec¢ (PHCP) has typical spectral properties previously observed for other cytochromesc¢, which comprise Ambler’s class II cytochromesc. The PHCP protein sequence (135 amino acids) deduced from the cloned gene is the most homologous (55% iden-tity) to that of cytochrome c¢ from Allochromatium vinosum(AVCP). | 1FEBS Journal Heterologous synthesis of cytochrome c by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery Hiroki Inoue1 Satoshi Wakai1 Hirofumi Nishihara2 and Yoshihiro Sambongi1 1 Graduate Schoolof Biosphere Science Hiroshima University Japan 2 Faculty of Agriculture Ibaraki University Japan Keywords cytochrome c biogenesis cytochrome c Escherichia coli heterologous synthesis System I Correspondence Y. Sambongi Graduate School of Biosphere Science Hiroshima University 1-4-4 Kagamiyama Higashi-Hiroshima Hiroshima 739-8528 Japan Fax 81 824 24 7924 Tel 81 824 24 7924 E-mail sambongi@ Received 28 February 2011 revised 5 April 2011 accepted 27 April 2011 doi Hydrogenophilus thermoluteolus cytochrome c PHCP has typical spectral properties previously observed for other cytochromes c which comprise Ambler s class II cytochromes c. The PHCP protein sequence 135 amino acids deduced from the cloned gene is the most homologous 55 identity to that of cytochrome c from Allochromatium vinosum AVCP . These findings indicate that PHCP forms a four-helix bundle structure similar to AVCP. Strikingly PHCP with a covalently bound heme was heterologously synthesized in the periplasm of Escherichia coli strains deficient in the DsbD protein a component of the System I cytochrome c biogenesis machinery. The heterologous synthesis of PHCP by aerobically growing E. coli also occurred without a plasmid carrying the genes for Ccm proteins other components of the System I machinery. Unlike Ambler s class I general cytochromes c the synthesis of PHCP is not dependent on the System I machinery and exhibits similarity to that of E. coli periplasmic cytochrome b562 a 106-residue four-helix bundle. Database The sequence data reported here have been deposited in the DDBJ database under accession no. AB617519. Introduction Cytochromes c are classified as class II cytochromes c according to Ambler 1 and are found in .

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