TAILIEUCHUNG - Báo cáo khoa học: Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein

Plant pathogenesis-related (PR) proteins of class 10 are the only group among the 17 PR protein families that are intracellular and cytosolic. Sequence conservation and the wide distribution of PR-10 proteins throughout the plant kingdom are an indication of an indispensable func-tion in plants, but their true biological role remains obscure. | Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein Humberto Fernandes1 Anna Bujacz2 Grzegorz Bujacz1 2 Filip Jelen3 Michal Jasinski1 Piotr Kachlicki4 Jacek Otlewski3 Michal M. Sikorski1 and Mariusz Jaskolski1 5 1 Institute of Bioorganic Chemistry Polish Academy of Sciences Poznan Poland 2 Institute of TechnicalBiochemistry Faculty of Biotechnology and Food Sciences TechnicalUniversity of Lodz Poland 3 Department of Protein Engineering Faculty of Biotechnology University of Wroclaw Poland 4 Institute of Plant Genetics Polish Academy of Sciences Poznan Poland 5 Department of Crystallography Faculty of Chemistry A. Mickiewicz University Poznan Poland Keywords cytokinin N N -diphenylurea plant hormones PR-10 proteins yellow lupine Correspondence M. Jaskolski Department of Crystallography Faculty of Chemistry A. Mickiewicz University Grunwaldzka 6 60-780 Poznan Poland Fax 48 61 829 1505 Tel 48 61 829 1274 E-mail mariuszj@ Received 3 December 2008 revised 8 January 2009 accepted 9 January 2009 doi Plant pathogenesis-related PR proteins of class 10 are the only group among the 17 PR protein families that are intracellular and cytosolic. Sequence conservation and the wide distribution of PR-10 proteins throughout the plant kingdom are an indication of an indispensable function in plants but their true biological role remains obscure. Crystal and solution structures for several homologues have shown a similar overall fold with a vast internal cavity which together with structural similarities to the steroidogenic acute regulatory protein-related lipid transfer domain and cytokinin-specific binding proteins strongly indicate a ligand-binding role for the PR-10 proteins. This article describes the structure of a complex between a classic PR-10 protein Lupinus luteus yellow lupine PR-10 protein of subclass 2 and N N -diphenylurea a synthetic cytokinin. Synthetic cytokinins have been shown in various

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