TAILIEUCHUNG - Báo cáo y học: "Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells. | Journal of Brachial Plexus and Peripheral Nerve Injury BioMed Central Research article Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells Erick O Fuentes1 Jost Leemhuis2 G Bjorn Stark1 and Eva M Lang 1 Open Access Address Department of Plastic and Hand Surgery University of Freiburg Medical Centre Freiburg Germany and institute of Experimental and Clinical Pharmacology and Toxicology Centre for Neuroscience Freiburg Germany Email Erick O Fuentes - ckitofdr@ Jost Leemhuis - G Bjorn Stark - Eva M Lang - evalang33@ Corresponding author Published 25 September 2008 Received 8 April 2008 Journal of Brachial Plexus and Peripheral Nerve Injury 2008 3 19 doi l 749-7221-3-19 Accepted 25 September 2008 This article is available from http content 3 1 19 2008 Fuentes et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system CNS substrates. Methods To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system PNS following nerve damage dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium C3 fusion toxin and the Rho kinase ROCK inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous . membrane only the group incubated with H1152

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