TAILIEUCHUNG - Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide

To learn more about the evolution of the cholinesterases (ChEs), acetylcho-linesterase (AChE) and butyrylcholinesterase in the vertebrates, we investi-gated the AChE activity of a deuterostome invertebrate, the urochordate Ciona intestinalis,by expressingin vitro a synthetic recombinant cDNA for the enzyme in COS-7 cells. | ỊFEBS Journal Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide A rw c rnfl zt t i ft lx1 I Z 1 f t Tq I Z 1 zt I n 2 c ro n ft c p i honrM c I Ixzt r1 c ri ft l roir 3 Adam rreueriuk Igoi isigeiny riaHUco Coiiciiuui Ciiiioiopiiei spikei Eiic Ixiejui Arnaud Chatonnet4 Stefan BoMigcm1 Gieg Richards1 Tessa Allen1 Maiy H. Whitlock1 and Leo Pezzementi1 1 Department of Biology Birmingham-Southern College Birmingham AL USA 2 Department of Chemistry and Biochemistry San Diego Supercomputer Center University of California at San Diego La Jolla CA USA 3 Institut Nationalde la Sante et de la Recherche Medicale U686 Universite Paris Descartes Biologie des Jonctions Neuromusculaires Paris France 4 Institut Nationalde la Recherche Agronomique Montpellier France Keywords acetylcholinesterase asymmetric forms butyrylcholinesterase Ciona intestinalis evolution Correspondence L. Pezzementi Department of Biology Birmingham-Southern College Box 549022 Birmingham AL 35254 USA Fax 1 205 226 3078 Tel 1 205 226 4806 E-mail lpezzeme@ Website http lpezzeme Database The nucleotide sequence and derived amino acid sequence data reported for the AChE from Ciona intestinalis are available in the Third Party Annotation Section of the DDBJ EMBL GenBank databases under the accession no. TPA BK006073. The alignment used to determine the phylogenetic tree for vertebrate and invertebrate cholinesterases presented here is deposited at the EMBL-ALIGN database as ALIGN_001208 Received 14 November 2007 revised 7 January 2008 accepted 15 January 2008 doi To learn more about the evolution of the cholinesterases ChEs acetylcholinesterase AChE and butyrylcholinesterase in the vertebrates we investigated the AChE activity of a deuterostome invertebrate the urochordate Ciona intestinalis by expressing in vitro a synthetic recombinant cDNA .

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