TAILIEUCHUNG - Báo cáo khoa học: Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae – involvement of the mitochondrial FAD transporter, Flx1p

The mitochondrial FAD transporter, Flx1p, is a member of the mitochon-drial carrier family responsible for FAD transport inSaccharomyces cerevi-siae. It has also been suggested that it has a role in maintaining the normal activity of mitochondrial FAD-binding enzymes, including lipoamide dehydrogenase and succinate dehydrogenase flavoprotein subunit Sdh1p. | ỊFEBS Journal Succinate dehydrogenase flavoprotein subunit expression in Saccharomyces cerevisiae - involvement of the mitochondrial FAD transporter Flx1p Teresa A. Giancaspero1 Robin Wait2 Eckhard Boles3 and Maria Barile1 1 Dipartimento di Biochimica e Biologia Molecolare E. Quagliariello Universita degli Studi di Bari Italy 2 Kennedy Institute of Rheumatology Division Faculty of Medicine ImperialCollege London UK 3 Institut fur Molekulare Biowissenschaften . Goethe-Universitat Frankfurt am Main Germany Keywords flavin Flx1p mitochondrialFAD transporter post-transcriptionalcontrol succinate dehydrogenase flavoprotein subunit Correspondence M. Barile Via Orabona 4 70126 Bari Italy Fax 39 0805443317 Tel 39 0805443604 E-mail Received 30 July 2007 revised 27 December 2007 accepted 4 January 2008 The mitochondrial FAD transporter Flxlp is a member of the mitochondrial carrier family responsible for FAD transport in Saccharomyces cerevi-siae. It has also been suggested that it has a role in maintaining the normal activity of mitochondrial FAD-binding enzymes including lipoamide dehydrogenase and succinate dehydrogenase flavoprotein subunit Sdh1p. A decrease in the amount of Sdh1p in the flxlD mutant strain has been determined here to be due to a post-transcriptional control that involves regulatory sequences located upstream of the SDH1 coding sequence. The SDH1 coding sequence and the regulatory sequences located downstream of the SDH1 coding region as well as protein import and cofactor attachment seem to be not involved in the decrease in the amount of protein. doi Several mitochondrial dehydrogenases and oxidases require FMN and FAD for their activity 1 2 . Thus intramitochondrial flavin cofactor availability is potentially a crucial regulator of oxidative terminal metabolism. Consistent with this some patients suffering from riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency RR-MADD .

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