TAILIEUCHUNG - Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila

The vestimentiferan annelidRiftia pachyptilaforms dense populations at hydrothermal vents along the East Pacific Rise at a depth of 2600 m. It harbors CO2-assimilating sulfide-oxidizing bacteria that provide all of its nutrition. To find specific host transcripts that could be important for the functioning of this symbiosis, we used a subtractive suppression hybridiza-tion approach to identify plume- or trophosome-specific proteins. | ỊFEBS Journal Identification sequencing and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila Sophie Sanchez Ann C. Andersen Stephane Hourdez and Francois H. Lallier Equipe Ecophysiologie Adaptation et Evolution Moleculaires UMR 7144 CNRS UPMC Station Biologique Roscoff France Keywords chemoautotrophy differential expression messenger RNA symbiosis Siboglinidae Correspondence F. H. Lallier Equipe Ecophysiologie Adaptation et Evolution Moleculaires UMR 7144 CNRS UPMC Station Biologique Place Georges Teissier BP 74 29682 Roscoff Cedex France Fax 33 29829 2324 Tel 33 29829 2311 E-mail lallier@ Database Nucleotide sequence data are available in the GenBank database under the accession numbers EF490380 RpCAbr and EF490381 RpCAbr2 Received 22 March 2007 revised 24 July 2007 accepted 20 August 2007 doi The vestimentiferan annelid Riftia pachyptila forms dense populations at hydrothermal vents along the East Pacific Rise at a depth of 2600 m. It harbors CO2-assimilating sulfide-oxidizing bacteria that provide all of its nutrition. To find specific host transcripts that could be important for the functioning of this symbiosis we used a subtractive suppression hybridization approach to identify plume- or trophosome-specific proteins. We demonstrated the existence of carbonic anhydrase transcripts a protein endowed with an essential role in generating the influx of CO2 required by the symbionts. One of the transcripts was previously known and sequenced. Our quantification analyses showed a higher expression of this transcript in the trophosome compared to the branchial plume or the body wall. A second transcript with nucleotide identity compared to the previous one was almost only expressed in the branchial plume. Fluorescent in situ hybridization confirmed the coexpression of the two transcripts in the branchial plume in contrast with the trophosome where only one .

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